Wasp Genomes are Sequenced, Revealing Surprises
News Jan 25, 2010
A consortium of more than 100 scientists has completed an analysis of the DNA sequence of three parasitic wasp species, and the project has turned up a few surprises in the process.
The genome project was led by University of Rochester and Baylor College of Medicine scientists, with Indiana University Bloomington biologists providing key genetic findings.
A Nasonia female is stinging a fly pupal host, injecting venom and laying her eggs within it. The offspring will emerge approximately two weeks later.
"We've gained a better appreciation for the diversity of genes that are found among the insects," said evolutionary biologist John Colbourne, who led IU's contribution. "There are likely 5 to 10 million species of insects and crustaceans on the globe that altogether show a staggering variety of methods to survive and reproduce. This project completes the initial description of the ninth distinct group of insects, and we find yet more novel features of the genome. Some can be matched to unique biological attributes."
Colbourne is the genomics director for IU's Center for Genomics and Bioinformatics.
The three wasp species are all members of the genus Nasonia -- vitripennis, giraulti, longicornis. And all three wasps sting and lay eggs within the larvae of agricultural pests as a food source for the wasps' young.
While digesting the vast information produced by the project (about 250 million base pairs per species, and tens of thousands of gene variants and proteins), consortium scientists found that the majority of Nasonia's genes are shared with humans and other animals, while one of every five genes belong to insects alone, and roughly two of every 100 genes are singularly shared between wasps and their close cousins, the honeybee.
Evidence is found for many instances of "lateral gene transfer," in which the wasps' genomes absorbed genetic material from viruses, bacteria, and other Nasonia species.
the scientists also identified a variety of important genes for the animal's way of life - genes involved in the production of venoms, genes likely responsible for the origin of each species, and genes that regulate the action of other genes in modifying DNA by adding (or removing) methyl groups.
By investigating the gene and gene transcripts that ultimately code for proteins that define a wide variety of wasp cell types, the scientists have named 79 genes that could be involved in the production and organization of wasp venoms. The scientists recognized some of these genes as similar to those found in other venom-producing insects, but about half of the 79 candidate venom genes seemed unique to the wasps. The venoms of Nasonia wasps are known to have diverse effects on their targets - from ataxia to paralysis to death.
The scientists examined the many subtle genetic differences between the three wasp species. They found distinct, correlated changes in nuclear and mitochondrial genes that seem to have occurred around the time of each species lineage's evolution, suggesting the coevolution of these nuclear and mitochondrial genes could have played a role in the origins of each species.
The wasps possess a full complement of methylation/de-methylation genes, which are used to modify nuclear chromosomes so that target genes are turned on or off. DNA methylation was once thought to be the exclusive property of mammals, but discoveries of methylation systems in wasps, honeybees, and other non-mammalian animals seem to suggest DNA methylation is likely to be far more common - and older - than once thought. Not all organisms possess the system. Fruit flies, for example, don't have it.
Nasonia wasps are used in agriculture to control insect pests. An understanding of the wasps' genetics could lead to tweaks in the wasps' DNA that improves their efficacy or specificity for particular pests (leaving other, beneficial insects alone). Nasonia giraulti and longicornis are native to North America, and vitripennis is found around the world.
Nasonia is the second hymenopteran insect genus to have its genome sequenced. Apis (mellifera, or honeybee), was the first. Like Apis, Nasonia females have two sets of chromosomes, while the males have only one. This split character in the insects' genetic identities can actually make studying them easier. By isolating active genes in males, scientists can more easily determine the purpose and qualities of those genes, in part because there is only one copy of those genes present - no second copy can interfere with the expression of the first, nor mask its effects. Just as importantly, the genetic systems of Nasonia and Apis simplify the breeding of genetically pure or near-pure lines.
Nasonia differs from Apis in important ways. For one, DNA methylation in Apis is known to help determine bees' caste status. Bees are true social insects, whereas Nasonia wasps live largely solitary lives.
Algorithm Speeds Up Medical Image Analysis 1000 TimesNews
Medical image registration is a common technique that involves overlaying two images, such as magnetic resonance imaging (MRI) scans, to compare and analyze anatomical differences in great detail. Researchers have described a machine-learning algorithm that can register brain scans and other 3-D images more than 1,000 times more quickly using novel learning techniques.
Mechanism Controlling Multiple Sclerosis Risk IdentifiedNews
Researchers at Karolinska Institutet have now discovered a new mechanism of a major risk gene for multiple sclerosis (MS) that triggers disease through so-called epigenetic regulation. They also found a protective genetic variant that reduces the risk for MS through the same mechanism.
Synthetic DNA Shuffling Enzyme Outpaces Natural CounterpartNews
A new synthetic enzyme, crafted from DNA rather than protein, flips lipid molecules within the cell membrane, triggering a signal pathway that could be harnessed to induce cell death in cancer cells. Researchers say their lipid-scrambling DNA enzyme is the first in its class to outperform naturally occurring enzymes – and does so by three orders of magnitudeREAD MORE