Pharmacophore Mapping of Flavone Derivatives for Aromatase Inhibiton
The study explores the structural requirements of flavones for inhibition of aromatase activity. The QSAR analysis generates the model that shows the importance of flavone ring and with molecular lipophilicity. Presence of additional aromatic ring and m-hydroxy substitution on that ring increases inhibitory activity. Space modeling study further adjudged the presence of hydrogen bond acceptor, hydrophobic and aromatic ring and critical distance among features for the inhibitory activity.
The Impact of the PCK1 Gene and PPCK1 Promoter Polymorphism 232C→G on the Incidence of TIIDM in Oji-Cree Natives of Ontario
The reported incidence of Type II Diabetes Mellitus (TIIDM) in Oji-Cree Natives is the highest in the world. This poster presents the impact of the PCK1 gene on TIIDM incidence in Oji-Cree Natives with particular attention to the role of SNP 232C→G on the transcription rate of phosphoenolpyruvate carboxykinease (PPCK1).
EasyBeacons™ - new Probes Ideal for Realtime PCR Detection of Methylation Status of Single CpG Duplets and SNPs
The EasyBeacons™ presented here are based on the novel technology Intercalating Nucleic Acid, INA®, linked to a fluorophore and a quencher. INA® is composed of normal DNA nucleotides and Intercalating Pseudo Nucleotides (IPNs). The fact that the EasyBeacons™ are mostly composed of normal DNA nucleotides means that in many respects EasyBeacons™ behave like DNA based probes, allowing use of standard buffers, primers and enzymes and hence reduces the optimisation efforts.
Novel Fluidics Microbead Trap/Flow Cell Enhances Speed/Sensitivity of Bead-Based Bioassays Up to 5-Fold
Pacific Northwest National Laboratory (PNNL) has developed a micro/nano particle trap that allows surface-functionalized magnetic or non-magnetic particles to be trapped with subsequent perfusion of sample, reagents and wash solutions, yielding significant (up to 5-fold) improvements in assay speed and sensitivity, while significantly reducing sample matrix effects.
Biomarker Identification Combining Multivariate Analysis of NMR Spectra with an Innovative Spectral Data Analysis Approach in an Integrated Working Environment
A recently developed Metabolomics informatics solution from raw data processing to metabolite database searching, compatible with multiple instruments, has been successfully applied to the analysis of 1H NMR human serum spectra of diabetic and non-diabetic subjects. This study also highlights Overlap Density Heatmap, a novel technology that complements the use of chemometrics in biomarker identification.
Toward Diagnosis of Diabetes by NMR and Mulitvariate Analysis: Study of 1H NMR Spectra of Human Serum in an Integrated Working Environment
In order to improve the efficiency of NMR-based metabolomics studies, we have developed KnowItAll® Informatics System, Metabolomics Edition as a consistent and integrated software environment that covers the entire process from processing raw NMR data —to multivariate analysis—to biomarker identification. Using two datasets of 1H NMR spectra of mouse urine and human serum, we demonstrate the high efficiency of this integrated workflow for metabolomics data analysis.
Combined Immune Parameters and X-ray data in Early Prediction of Anti-Tuberculosis Chemotherapy Response
20 tuberculosis (12 slow-responders and 8 fast responders) patients were treated with directly observed short course anti-tuberculosis chemotherapy. Chest X-ray was performed. sICAM-1 and suPAR were measured in serum by ELISA, TNFRs using the luminex technology. General discrimination analysis on selected analytes gave, 91.66% and 87,50% correctly classify fast responders and slow responder respectively. The support vector machine analysis gave 100% correct classification.
Miniaturisation of Liquid Handling Procedures for High Throughput Sequencing
Deerac Fluidics Equator™ products are designed to fit a wide range of applications requiring low volume liquid handling. The products have recently been installed as part of the high throughput sequencing production process at the Broad Institute in Boston, MA, USA. This poster will describe the current process employed by Broad Institute, highlighting where the Equator™ has played a vital role in performance enhancement and cost reduction, enabling the Broad Institute to retain its position as