Identification and Characterization of Strain Specific Drug Target by Subtractive Genome Analysis of Methicillin Resistance Staphylococcus aureus
Poster Feb 20, 2013
Kiran Saeed and Reaz Uddin
In the present study, the idea was to utilize the strain specific available genome or proteome by using ‘subtractive genomics approach’ which helped in the identification and characterization of strain specific drug targets along with comparative analysis within two of the Methicillin resistance Staphylococcus aureus (MRSA) strains i.e., MRSA398 and MRSA252.The protocol involved various bioinformatics’ tools and databases like similarity search between pathogen and host, essentiality study using the database of essential genes, metabolic functional association study using Kyoto Encyclopedia of Genes and Genomes Database (KEGG), cellular membrane localization analysis, drug bank database.
When there is a need to quickly analyze samples using a number of different PCR assays, it is likely that optimal conditions for each assay will not be the same. First, different assays often will require different annealing temperatures for their primers. In addition, amplicons may be designed to be of different lengths and therefore require varying durations of the extension step.READ MORE