TMT Quantification for Shotgun Proteomics: TIMS-QTOF Powered by PASEF
Poster May 31, 2019
Matt Willetts, Shourjo Ghose, Heiner Koch, Scarlet Koch, Markus Lubeck, Gary Kruppa.
This poster evaluates the performance of ‘timsTOF Pro’ a unique dual trapped ion mobility spectrometer (TIMS) coupled to a QTOF for the analysis of isobaric labelling experiments.
Download to discover:
- How TIMS provides an additional dimension of separation and high peak capacity
- Why the timsTOF Pro instrument is well suited to isobaric labelled quantitation experiments
- How to achieve near 100% duty cycle for shotgun proteomics
Basic fibroblast growth factor (bFGF) is widely used in vitro for the maintenance and stimulation of a variety of cells. However, use of native bFGF in cell biology is limited by the fact that bFGF rapidly degrades at physiological temperatures. We have addressed this problem with an engineered form of bFGF, named Heat Stable bFGF (HS bFGF), which is stable at 37 degrees Celsius.READ MORE
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