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Enhancing Efficiency in Multi-scale, Multi-modal Workflows For 3D Tomography

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Materials and life science researchers can now experience faster accessibility of deeper regions of interest when investigating samples in 3D. New extensions on ZEISS Crossbeam 350/550 and ZEISS Atlas 5 offer improvements in speed and data quality when performing multi-scale, multi-modal studies in additive manufacturing, electronics, battery research, biomaterials and biological tissue investigations on resin-embedded biological specimens.

Researchers who perform multi-scale, correlative studies using X-ray microscopy data to drive FIB-SEM site selections can further enhance their workflows: the newly introduced, patented LaserFIB on ZEISS Crossbeam 350/550 is designed to enable the access of deeply buried structures in addition to rapid, gallium free structure fabrication over large length scales. Users can perform laser work in a dedicated chamber to avoid contamination of the main instrument. A next generation femtosecond laser added to the airlock provides massive material ablation in a short time while virtually eliminating laser-induced heat effects.

The LaserFIB can be used to produce structures such as cantilevers, pillars for nanomechanical testing and for sample preparation in the ZEISS Xradia Ultra X-ray microscope. Further applications are the creation of large cross sections for EBSD (electron backscattered diffraction) studies or whole TEM grids with guaranteed gallium free results.

ZEISS has also introduced extensions of ZEISS Atlas 5 for imaging, 3D Tomography and a toolkit for analysis and reporting. Enhanced correlative workflows are now included due to improvements in relocation of regions of interest when guiding LaserFIB or FIB-SEM workflows using X-Ray microscopy data. Users can take advantage of relocating regions of interest faster and more accurately. Once a region has been located, thin & fast tomography takes over. This is a method for tomogram acquisition, slice thickness measurement and improved visualization and data processing with ZEISS’ patented True-Z technology. True-Z measures each slice thickness in turn and reconstructs the tomogram accounting for variability in z slice thickness. This can provide greater accuracy for the final reconstructed volume making 3D and 4D modeling more accurate and increasing precision of segmentation when investigating 3D volumes.

Correlated data of a multi-modal dataset are visible at one glance with simultaneous visualization of up to four imaging or analytic modalities. Storyboarding, the production of curated slideshows and the possibilities of digital education are facilitated by ZEISS’ new Enhanced Browser-Based Viewer Export Module.