In-Fusion® Snap Assembly EcoDry™ Master Mix

In-Fusion® Snap Assembly EcoDry™ products feature our In-Fusion Snap Assembly master mix in a lyophilized, ready-to-use format that can be stored at room temperature. The cloning reagent is prealiquoted and dried down in tubes or well-plates that are covered with an aluminum seal. The room temp stability makes this convenient cloning enzyme format ideal for high-throughput cloning with robotic liquid handlers. Whether at the bench or with automation platforms, use of pre-aliquoted reagents means minimal handling—eliminating chances of contamination, pipetting errors, or missing reagents. Just like its liquid counterpart, In-Fusion Snap Assembly EcoDry allows you to clone any PCR fragment into any linearized vector in a single step without restriction digestion of the PCR fragment, ligation, or blunt-end polishing.

The ability to clone directly into any vector at any restriction site eliminates the need for further subcloning or manipulation, dramatically simplifying the cloning workflow and enabling transition to high-throughput applications. In-Fusion Snap Assembly EcoDry master mix fuses PCR-generated sequences and linearized vectors efficiently and precisely, utilizing a 15-bp overlap at their ends. This 15-bp overlap can be engineered by designing custom primers for amplification of the desired sequences using our free primer design tool.

In-Fusion Cloning technology is based on the removal of nucleotides from the 3' end of the linear DNA strands. This allows complementary base pairs between two fragments of DNA to anneal, leading to fragment joining. This method can be used to clone single or multiple fragments into a single vector without subcloning. Additional applications include directional cDNA cloning, mutagenesis, gene synthesis, high-throughput cloning, as well as the addition of adaptors, linkers, and protein tags.