Automation – Multimedia

This poster will describe the current process employed by KBiosciences. The vital role spot-on™ technology, incorporated into the Equator™ benchtop robotic liquid handling instrument, has played in improving performance and reducing costs are highlighted.

In the proteomics area with the increasing capacity of the mass spectrometers, sample preparation is becoming a limiting step for protein identification. Magnetic particle based technology provides a rapid and easy solution for automation of the sample preparation step.

The drive towards miniaturization within the pharmaceutical and biotechnology fields has created a need for liquid handling technologies that accurately deliver low volume reagents to high-density plates. This has also created a need for simple, fully scaleable assays in low volumes. Here we demonstrate the successful combination of both through the use of the Equator™ NS 808 - Eight Tip Pipetting System and the dual color Chroma-Luc™ technology.

Deerac Fluidics Equator™ products are designed to fit a wide range of applications requiring low volume liquid handling. The products have recently been installed as part of the high throughput sequencing production process at the Broad Institute in Boston, MA, USA. This poster will describe the current process employed by Broad Institute, highlighting where the Equator™ has played a vital role in performance enhancement and cost reduction, enabling the Broad Institute to retain its position as

Using the Muscarinic M1 G-protein Coupled Receptor as a model system, we achieved accurate and reproducible determinations of binding affinity (Kd) and IC50 results for known ligands on the 384-well plate device. As a result of being able to incubate the reaction mixture in the filter plate and configure the assay using half the reaction volume, significant reductions in reagent costs and radioactive waste were achieved.