Comprehensive Profiling of miRNAs in Stem Cells Using Microfluidics
News Oct 06, 2009
MicroRNAs (miRNAs) have been shown to be key regulators of hematopoiesis. However, the detailed profile of miRNA expression throughout hematopoietic development has not been charted, owing primarily to challenges in obtaining highly purified samples of stem and progenitor cells as well as profiling hundreds of miRNAs from low cell numbers. To overcome these limitations, the new technical poster describes how microfluidic technologies were combined with a 288-plex real time PCR approach, allowing the detection of 288 miRNAs in small numbers (c. 3000) of cells.
The poster describes how over twenty unique murine hematopoietic cell populations were isolated through FACS sorting, including hematopoietic stem cells (HSCs), myeloid and lymphoid progenitor cells and mature lineage populations. Several thousand nanolitre volume quantitative PCR reactions were performed using Fluidigm BioMark™ 48.48 Dynamic Arrays and miRNA specific TaqMan® probes. For each miRNA, synthetic miRNA standards were used to determine the absolute number of miRNA molecules per cell. 133 of the 288 miRNAs tested were detected across all cell populations.
The researchers conclude that Fluidigm's dynamic array provides a rapid, sensitive and cost-effective method for miRNA profiling in rare cell populations. The sensitivity of Fluidigm's dynamic array is shown to detect heterogeneity in miRNA expression at the single cell level in purified populations.
A copy of the technical poster is available on request by contacting email@example.com
“Nanokicking” Technology Enables Breakthrough in the Culture of 3D Mineralised Bone TissueNews
Researchers have grown 3D samples of mineralised bone from human mesenchymal stem cells for the first time without typical osteoinductive media.READ MORE
Methane Hydrate Formation Studied Using Novel MicroreactorNews
Researchers at the NYU Tandon School of Engineering are using a novel means of studying how methane and water form methane hydrate that allows them to examine discrete steps in the process faster and more efficiently.READ MORE
Lissencephaly Modeled in a Dish for the First TimeNews
Researchers describe how cells from patients with the severe developmental disease lissencephaly differ from healthy cells. The method can provide vital new knowledge on difficult-to-study congenital diseases.READ MORE
Comments | 0 ADD COMMENT
9th International conference on Tissue Engineering and Regenerative Medicine
Nov 09 - Nov 10, 2018
International Conference & Exhibition on Tissue Engineering and Biobanking
Apr 23 - Apr 24, 2018