Density-Dependent Separation of Encapsulated Cells in a Microfluidic Channel
News Jun 13, 2012
This study presents a method for density-based separation of monodisperse encapsulated cells using a standing surface acoustic wave (SSAW) in a microchannel. Even though monodisperse polymer beads can be generated by the state-of-the-art technology in microfluidics, the quantity of encapsulated cells cannot be controlled precisely. In the present study, mono-disperse alginate beads in a laminar flow can be separated based on their density using acoustophoresis. A mixture of beads of equal sizes but dissimilar densities was hydrodynamically focused at the entrance and then actively driven toward the sidewalls by a SSAW. The lateral displacement of a bead is proportional to the density of the bead, i.e., the number of encapsulated cells in an alginate bead. Under optimized conditions, the recovery rate of a target bead group (large-cell-quantity alginate beads) reached up to 97% at a rate of 2300 beads per minute. A cell viability test also confirmed that the encapsulated cells were hardly damaged by the acoustic force. Moreover, cell-encapsulating beads that were cultured for 1 day were separated in a similar manner. In conclusion, this study demonstrated that a SSAW can successfully separate monodisperse particles by their density. With the present technique for separating cell-encapsulating beads, the current cell engineering technology can be significantly advanced.
This article was published online in Biomicrofluidics and is free to access.
Dielectrophoresis Chromatography Could Advance Laboratory AnalysisNews
Research being done at Rochester Institute of Technology to refine lab-on-chip devices—highly sophisticated laboratories on microchips—will provide more detailed and timely information to detect diseases such as cancer.READ MORE
New Test Reveals Antibiotic-Resistant Bacteria in 30 MinutesNews
The new procedure shortens wait times from three days and could help reduce the spread of superbug bacteria.READ MORE