Latest Application Notes

Single cell analysis in a spatially resolved context is the gateway to deciphering scientific questions in various application areas. Such analysis requires the highest available spatial precision combined with highly sensitive detection.

Highly ordered protein aggregates, termed amyloid fibrils, are associated with a broad range of diseases, many of which are neurodegenerative for example Alzheimer’s and Parkinson’s. The transition from soluble, functional protein to insoluble amyloid fibril occurs via a complex process involving the initial generation of highly dynamic early stage aggregates or prefibrillar species.

The specificity of fluorescence microscopy allows researchers to accurately observe and analyze biological processes and structures quickly and easily, even when using thick or large samples. However, out-of-focus fluorescence increases background, reduces contrast and makes accurate image segmentation more challenging.

Spatially resolved gene expression can provide a powerful complement to traditional histopathology methods, enabling a greater understanding of cellular heterogeneity and organization within the central nervous system.

Studying tissue and organ biology is challenging, particularly in mammals, given sample accessibility and ethical concerns. However, the use of organoids alleviates these challenges, supporting researchers in exploring questions that previously could not have been investigated without them.

Functional genetic screening using the CRISPR/Cas9 system is a powerful tool for interrogating gene function at scale, but access to and implementation of this technology is constrained by existing pre-defined CRISPR sgRNA libraries.

Download this research snapshot to discover how immune repertoire changes in patients with age-related neurodegeneration and how adaptive immune cells are involved in these neurodegenerative diseases.

Download this app note to learn more about high-throughput single-cell RNA sequencing and how it can be used to analyze cells and tissues in a variety of ways.

Interrogation of complex tissue types requires a highly sensitive, specific, and multiplexed spatial approach with single-cell resolution - download this app note to discover a technology that rises to this challenge.

Agilent Seahorse XF technology measures energy metabolism in live cells, providing critical information that relates directly to cellular function. Download this application note to discover how the Seahorse XF assay can be used to predict cancer cell vulnerabilities, immune cell fate and function and neuronal cell health.