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Application Notes

 
Forensic Investigations using Confocal Raman Imaging
Application Note

WHEATON

Confocal Raman Imaging is a very effective tool for forensic analysis as it provides information on the chemical composition.

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General V3 Western Blotting Protocol
Application Note

Bio-Rad Laboratories

Western blotting is a very useful and widely adopted lab technique, but the traditional procedure can be long and tedious. Researchers can assess only whether a blot image is captured at the end of the long procedure and the quality of western blot data is sometimes challenged due to poor loading controls.

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Avoiding Housekeeping Protein Detection Saturation
Application Note

Bio-Rad Laboratories

Reliable western blot data require detection of the target and loading control proteins in the linear dynamic range. Many published western blot images show saturated protein bands, indicating that they were not measured in the linear dynamic range. Quantitative analysis based on this type of data is not reliable.

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Identification of the Site of the Octanoic Acid Post-Translational Modification in Human Ghrelin Using Electron Transfer Dissociation
Application Note

Washington University School of Medicine

ETD is a powerful fragmentation technique that has proved particularly useful for the determination of labile post-translational modifications of peptides and proteins. The technique has been used to determine the site-specific octanoylation of the peptide Ghrelin which could not be determined using Collision Induced Dissociation.

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ESGRO-2i Medium: Inhibitor-Based Serum-free Medium for ES and iPS Cell Culture
Application Note

EMD Millipore

Defined serum-free and feeder-free culture of mouse embryonic stem (mES) cells holds many advantages over the classical serum-containing feeder-dependent culture methods, ranging from decreased lot-to-lot variations to ease of culture.

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Kv1.5 Potassium Channel Assay Using the IonWorks HT System
Application Note

Molecular Devices

This application note describes the use of the IonWorks HT Platform with a voltagedependent potassium channel Kv1.5.

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Dual-Glo Luciferase Reporter-Gene Assay on the SpectraMax M5, LMax II384 and Analyst GT Microplate Readers
Application Note

Molecular Devices

Reporter-gene assays are used to study eukaryotic gene expression. Dual genetic reporters are commonly used in transient transfections of cultured cells to minimize experimental variability. Recently, Promega introduced a Dual-Glo Lucoferase assay system which replaced the outdated ‘flash’ assays used previously. Ensuring both genetic reporters have different substrate requirements enables them to be detected using a single reaction mixture.

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Recording of ICRAC in RBL-1 Cells on the PatchXpress 7000A Automated Patch Clamp System
Application Note

Molecular Devices

Calcium release-activated calcium (CRAC) channels play an important role in intracellular Ca2+ homeostasis. Like other store-operated calcium channels, currents through the CRAC channel (ICRAC) are activated by depletion of calcium in the endoplasmic reticulum (ER) and serve the purpose of slowly replenishing the ER with Ca2+.

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A Fast and Simple Method For Measuring P-Glycoprotein (Pgp) Inhibition
Application Note

BMG Labtech

Pgp expression has been linked to the effl ux of chemotherapeutic
drugs in human cancers leading to multidrug resistance. Pgp
activity can also result in low oral absorption and poor brain
penetration. Interaction of drugs with the Pgp may also cause
an increase in toxicity of co-administered compounds.

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Cellular Dopamine and Intracellular Calcium Signaling Using the Next Generation HTS Microplate Reader
Application Note

BMG Labtech

Two cell-based signaling assays from Invitrogen, Fluo-4 Direct™ Calcium Assay and Tango™-bla U2OS GPCR Assay, were performed on the PHERAstar FS HTS instrument. The PHERAstar FS has several unique features that enhance the performance of these assays including direct optic bottom reading, high resolution cell layer scanning, injection at the point of measurement and dual emission detection.

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