Latest Application Notes
3D cell culture: a realistic and reproducible collagen-based environment
Researchers are increasingly turning to 3D cell culture techniques, due to the improved physiological relevance of the cellular environment. 3D cell cultures offer a more in vivo-like response to stimuli more closely resembling the behavior of animal models or the human body.
Optimized cell culture
Culturing of various stable cell lines, including bacterial, yeast, hybridoma, insect and mammalian lines, and stem cells, is an important task in the pharmaceutical industry. The cells need to be maintained and expanded for use in cell-based assays, such as siRNA screening, ADME and high content screening, as well as for clone selection.
Forensic Investigations using Confocal Raman Imaging
Confocal Raman Imaging is a very effective tool for forensic analysis as it provides information on the chemical composition.
General V3 Western Blotting Protocol
Western blotting is a very useful and widely adopted lab technique, but the traditional procedure can be long and tedious. Researchers can assess only whether a blot image is captured at the end of the long procedure and the quality of western blot data is sometimes challenged due to poor loading controls.
Avoiding Housekeeping Protein Detection Saturation
Reliable western blot data require detection of the target and loading control proteins in the linear dynamic range. Many published western blot images show saturated protein bands, indicating that they were not measured in the linear dynamic range. Quantitative analysis based on this type of data is not reliable.
Identification of the Site of the Octanoic Acid Post-Translational Modification in Human Ghrelin Using Electron Transfer Dissociation
ETD is a powerful fragmentation technique that has proved particularly useful for the determination of labile post-translational modifications of peptides and proteins. The technique has been used to determine the site-specific octanoylation of the peptide Ghrelin which could not be determined using Collision Induced Dissociation.
ESGRO-2i Medium: Inhibitor-Based Serum-free Medium for ES and iPS Cell Culture
Defined serum-free and feeder-free culture of mouse embryonic stem (mES) cells holds many advantages over the classical serum-containing feeder-dependent culture methods, ranging from decreased lot-to-lot variations to ease of culture.
Kv1.5 Potassium Channel Assay Using the IonWorks HT System
This application note describes the use of the IonWorks HT Platform with a voltagedependent potassium channel Kv1.5.
Dual-Glo Luciferase Reporter-Gene Assay on the SpectraMax M5, LMax II384 and Analyst GT Microplate Readers
Reporter-gene assays are used to study eukaryotic gene expression. Dual genetic reporters are commonly used in transient transfections of cultured cells to minimize experimental variability. Recently, Promega introduced a Dual-Glo Lucoferase assay system which replaced the outdated ‘flash’ assays used previously. Ensuring both genetic reporters have different substrate requirements enables them to be detected using a single reaction mixture.
Recording of ICRAC in RBL-1 Cells on the PatchXpress 7000A Automated Patch Clamp System
Calcium release-activated calcium (CRAC) channels play an important role in intracellular Ca2+ homeostasis. Like other store-operated calcium channels, currents through the CRAC channel (ICRAC) are activated by depletion of calcium in the endoplasmic reticulum (ER) and serve the purpose of slowly replenishing the ER with Ca2+.