Detection of NADH and NADPH with the Omega’s High Speed, Full UV/Vis Absorbance Spectrometer
Application Note Mar 31, 2009
NAD+, NADH as well as NADP+, NADPH have been known to play vital roles in energy metabolism, antioxidation, and reductive biosynthesis. Changes in these systems can be monitored using absorbance spectroscopy. NADH and NADPH absorb light at 340 nm, whereas NAD+ and NADP+ do not. The FLUOstar, POLARstar and SPECTROstar Omega microplate readers from BMG LABTECH all have a UV/Vis spectrometer that can measure any absorbance range from 220-850 nm.