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Application Note

Normalization in Western Blotting to Obtain Relative Quantitation

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Application Note

Normalization in Western Blotting to Obtain Relative Quantitation

Normalization is a critical step in obtaining reliable and reproducible quantitative western blotting.

Under ideal conditions, normalization would not be necessary, but factors such as sample loading and transfer efficiency make normalizing the western blot essential.

Download this technical note to discover:

  • The basic principles of normalization using internal loading controls
  • How to accurately normalize your western blots to obtain meaningful, reproducible data
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