Transcreener® ADP2 TR-FRET Red Assay
Application Note Mar 12, 2014
Tecan Group Ltd.
The assay is based on the competitive binding of ADP to a monoclonal antibody-terbium conjugate, and uses a homogeneous TR-FRET detection mechanism. In the absence of free ADP, excitation of terbium results in energy transfer to a far-red tracer and emission at 665 nm.
Any ADP produced by the enzyme of interest displaces the tracer from the antibody, disrupting the FRET reaction
The Infinite F200 PRO, Infinite F500 and Infinite M1000 PRO multimode readers were tested for their capacity to measure the Transcreener ADP2 TR-FRET Red Assay.