In cell molecular sieve from protein crystal

Scientists at Tokyo Institute of Technology, RIKEN, and Kyoto Institute of Technology have applied rational crystal design to create protein crystals with extended porous network to accumulate exogenous molecules inside living cells. This work lays a foundation for engineering of stable self-assembling crystalline porous materials which can concentrate and preserve bioactive substances in various cell types.

Figure. Increase of PhC porosity by deletion of amino acids located on the molecular interfaces (top panel). The mutant polyhedrin monomers were crystallized in insect cells and used to adsorb fluorescent dyes (bottom panel).

In nature, proteins are assembled into sophisticated and highly ordered structures, which enable them to execute numerous functions supporting different forms of life. The exquisite design of natural proteins prompted scientists to exploit it in synthetic biology to engineer molecules that can self-assemble into nanoparticles with desired structure and that may be used for various purposes such as gas storage, enzyme catalysis, intracellular drug delivery, etc.

Cytoplasmic polyhedrosis viruses (cypoviruses) infecting insects are embedded in protein crystals called polyhedra which shield the virus from damage. The structure of polyhedra crystals (PhCs) suggests that they can serve as robust containers which can incorporate and protect foreign molecules from degradation, ensuring their compositional and functional stability.

Overview of Research Achievement

Extreme stability of polyhedra under harsh conditions is provided by dense packing of polyhedrin monomers in crystals with solvent channels of very low porosity, which, however, limits the incorporation of foreign particles. Research group led by Satoshi Abe and Takafumi Ueno at Tokyo Institute of Technology hypothesized that if a porous framework inside PhCs is extended without compromising crystal stability, PhCs can be used for accumulation and storage of exogenous molecules in living cells. As in natural PhCs, polyhedrin monomers form a trimer, the scientists assumed that if amino acid residues at the contact interface of each trimer are deleted, the porosity of the resulting crystals would be increased. To achieve this goal, they genetically engineered polyhedrin monomers, which were then expressed and self-assembled in Spodoptera frugiperda IPLB-Sf21AE, the larva of an armyworm moth, infected with baculovirus. The mutant PhCs maintained crystal lattice of the wild-type PhC but had significantly extended porosity (Figure) due to the deletion of amino acid residues with the rearrangement of intra- and intermolecular hydrogen bonds. As a result, the engineered crystals could adsorb 2-4 times more exogenous molecules (fluorescent dyes) compared to the wild type PhC, with up to 5,000-fold condensation of the dyes from the 10 uM solution.

As a next step, the scientists examined the performance of the mutant crystals in living insect cells. PhCs showed high stability in the intracellular environment. Most importantly, the mutant crystals could accumulate and retain the dyes in live cells, while the natural crystals could not.

Rationale crystal design used by scientists at Tokyo Institute of Technology provides a powerful tool for structural manipulation of self-assembled protein crystals to obtain porous nanomaterials with regulated adsorption properties. The engineered porous PhCs can be used as protein containers for in vivo crystal structure analysis of the cellular molecules and bioorthogonal chemistry in various types of living cells.

Structural analysis of microcrystals

Since tiny crystals with only a few microns size were obtained, the structure analyses were performed at beamlines BL32XU and BL41XU at SPring-8, a large synchrotron radiation facility which delivers the most powerful synchrotron radiation. The high-resolution structures were rapidly analyzed with the help of an automated data collection system developed in RIKEN.

Reference

Authors:	Satoshi Abe1, Hiroyasu Tabe2, Hiroshi Ijiri1, Keitaro Yamashita3, Kunio Hirata3, Kohei Atsumi1, Takuya Shimoi1, Masaki Akai1, Hajime Mori4, Susumu Kitagawa 2, 5, and Takafumi Ueno1
Title of original paper:	Crystal Engineering of Self-Assembled Porous Protein Materials in Living Cells
Journal:	ACS Nano
DOI :	10.1021/acsnano.6b06099
Affiliations :	1School of Life Science and Technology, Tokyo Institute of Technology 2Graduate School of Engineering, Kyoto University 3SR Life Science Instrumentation Unit, RIKEN/SPring-8 Center 4Centers for Advanced Insect Research Promotion, Kyoto Institute of Technology 5Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University

• Press release "In cell molecular sieve from protein crystal"
• Ferritin releases carbon monoxide in regulated therapeutic doses | Tokyo Tech News
• Protein-engineered cages aid studies of cell functions | Tokyo Tech News
• Ueno Laboratory
• Researcher Profile | Tokyo Tech STAR Search - Satoshi Abe
• Researcher Profile | Tokyo Tech STAR Search - Takafumi Ueno
• Department of Life Science and Technology, School of Life Science and Technology
• Latest Research News