Imaging mass spectrometry (IMS) requires the application of special sample pretreatment methods to enable the visualization of the target compounds with high sensitivity and high resolution.

The techniques that enable this visualization are the two-step matrix application and on-tissue derivatization methods. This TechNote describes an IMS analysis approach that uses these techniques to visualize corticosterone on rat adrenal tissue.

Download this TechNote to discover:

• the fundamentals of on-tissue derivatization
• how to increase IMS sensitivity and resolution
• how on-tissue derivatization can be used to visualize corticosterone, identify structural isomers, and enable successful IMS analysis