A representative video of the 3D visualization of blood vessels in the whole uteroplacental complex in control and DHT+INS-exposed rats. To label blood vessels within the uteroplacental complex, whole tissue together with the foetus was dissected, cleared with the iDISCO method, immunolabelled with anti-CD31 (red) and anti-α-SMA (green), and imaged by light-sheet fluorescence microscopy.
Read the Open Access article for free: Defective uterine spiral artery remodelling and placental senescence in a pregnant rat model of polycystic ovary syndrome.