Agilent Technologies and Anderson Forschung Group Collaborate on Quantitative Protein Assays
News Jun 03, 2009
Agilent Technologies Inc. and Anderson Forschung Group LLC (AFG) have announced a collaboration to develop quantitative peptide assays to speed protein biomarker discovery and validation.
The collaboration will combine AFG’s stable isotope standards and capture by anti-peptide antibodies (SISCAPA) technology with Agilent’s 1200 Series HPLC-Chip and 6400 Series triple quadrupole mass spectrometers (MS).
The combination will be used to develop methods for measuring the amounts of large numbers of peptides in digests of complex samples such as plasma. The results will benefit both organizations, and financial details were not disclosed.
“We’re proud to be partnering with a thought leader like Dr. Anderson to develop improvements in clinical mass spectrometry,” said Gustavo Salem, Agilent vice president and general manager, Biological Systems Division. “One of the greatest challenges to delivering useful knowledge using the protein biomarker discovery paradigm is achieving effective, reproducible and highly sensitive peptide quantitation.”
“SISCAPA assays for candidate biomarkers can benefit substantially from the reproducibility and sensitivity of Agilent’s platform, and we look forward to optimizing this combination,” said Leigh Anderson, AFG’s chief executive officer.
The SISCAPA method utilizes antibody-coated magnetic beads and a rotary magnetic bead trap device to capture target peptides, which are then, measured using a nanoflow LC-MS/MS system. The goal is to measure the quantities of very small amounts of peptides in sample digests, creating a useful research tool with potential uses in advanced diagnostics.
The Agilent 1200 Series HPLC-Chip/MS system is a microfluidic platform that integrates LC columns, connection capillaries, and nanospray emitter in a polymer chip, providing uncompromised chromatographic performance, even with small sample input amounts. The credit card-sized device is inserted into Agilent’s HPLC-Chip Cube to interface with the MS. Chip loading, solvent and sample delivery, high pressure switching of flows, and chip positioning in the MS source are all automated.
The Agilent 6400 Series triple quadrupole LC/MS systems can provide femtogram-level sensitivity across a broad mass range. These instruments have earned a reputation for reliably quantitating trace organic compounds in complex matrices, such as drug metabolites, herbicide levels in food and contaminants in ground water.
'Royal' Pheromone Identified in TermitesNews
Researchers have for the first time identified a specific chemical used by the higher termite castes – the queens and the kings – to communicate their royal status with worker termites. The findings could advance knowledge of termite evolution, behavior and control.READ MORE
Making Fragrances Last LongerNews
From floral perfume to fruity body wash and shampoos, scents heavily influence consumer purchases. But for most, the smell doesn’t last long after showering before it fades away. Scientists have now developed a way to get those fragrances to stick to the skin longer instead of washing down the drain immediately after being applied.READ MORE
Auto Industry Tip Improves Smoked FoodNews
Infusing foods with smoke can impart delicious flavors, but could also come with an unwelcome side of carcinogens. To reduce the carcinogen content of smoked foods, researchers took a lesson from the automobile industry, running the smoke through a zeolite filter to remove harmful compounds. It worked, and with a happy bonus: superior smoke flavor.READ MORE