Could 2D Mass Spec Breakthrough Lead to Medical Revolution?
News Oct 17, 2016
Professor Peter O’Connor and Dr Maria van Agthoven in the Department of Chemistry have invented a device which makes 2D mass spectrometry - an effective process for analysing and sequencing proteins - widely accessible for the first time ever.
This could lead to a revolution in the pharmaceutical and biomedical communities, enabling researchers and companies to produce data-driven results on how protein molecules function, more easily and cheaply.
2D mass spectrometry allows chemists to explore the elemental composition and structure of a molecule by breaking it apart, and analysing its fragmented pieces – measuring mass, and gathering data on how the whole molecule functions and interacts with its environment.
High numbers of molecules can be experimented on at the same time in this way, as the various fragments of different broken molecules can be modulated at the same frequencies as the molecule from which they originated.
Professor O’Connor and Dr van Agthoven have patented an instrument with which 2D Mass spectrometry can be performed using a linear ion trap – this is a cheaper, smaller, and much more accessible option than was previously available.
The device can be added onto existing MS instruments as well as being bought with new instruments.
The process is currently undertaken using large, expensive machinery, which makes it a relatively exclusive scientific activity. However, the new invention is opening up the technique to a much wider market.
Mass spectrometry produces precise results during protein sequencing, and this type of data-driven biology will produce quicker, better results than are currently obtained in pharmaceutical and biomedical research.
Dr van Agthoven comments that the breakthrough could have numerous and varied applications:
“Two-dimensional mass spectrometry has the potential to exponentially increase our knowledge in all areas, from biochemistry to food safety and environmental chemistry.”
Professor O’Connor is confident that this invention will change biomedical research dramatically:
"2-Dimensional mass spectrometry in a simple and cheap linear ion trap will revolutionise proteomics and detailed characterisation of complex samples."
Using EBX reagents, researchers have converted the C-terminal carboxylic acid of peptides into a carbon-carbon triple bond - an alkyne (in chemical jargon a "decarboxylative alkynylation"). The alkyne moiety is a very valuable functional group that can be used to further modify the peptides.READ MORE