Discover the Mysteries of the Brain with Applied Biosystems iTRAQ Reagents
News Nov 24, 2005
Researchers at the Centre for Neurogenomics and Cognitive Research, Vrije Universiteit, in The Netherlands, have announced that they are using Applied Biosystems iTRAQ reagent kits to analyse proteins in brain synapses using tandem mass spectrometry (MS/MS) on the Applied Biosystems 4700 Proteomics Analyzer.
The team are characterising the dynamic changes taking place in the synapse during the changing conditions involved in memory creation, learning and behaviour.
Dr Ka Wan Li, Assistant Professor, explained, "There are about 2000 different proteins in the synapse and they play major roles in brain function and plasticity."
"Unfortunately, many of them are membrane proteins, which cannot be easily separated on 2D gels. Instead we solubilise, digest and label them with iTRAQ reagents, then analyse them on the 4700 Proteomics Analyzer."
"The iTRAQ reagents label all the peptides in four different samples, which means we do 10,000 or more MS/MS mass spectra per experiment."
"Using the iTRAQ reagents with the 4700 Proteomics Analyzer has been a turning point in our research because it means we can perform quantitative analyses of the synapse proteome on a routine basis."
Analytical Tool Predicts Disease-Causing GenesNews
Predicting genes that can cause disease due to the production of truncated or altered proteins that take on a new or different function, rather than those that lose their function, is now possible thanks to an international team of researchers that has developed a new analytical tool to effectively and efficiently predict such candidate genes.
‘Good Cholesterol’ May Not Always be Good for Postmenopausal WomenNews
Postmenopausal factors may have an impact on the heart-protective qualities of high-density lipoproteins (HDL) – also known as ‘good cholesterol’ – according to a study led by researchers in the University of Pittsburgh Graduate School of Public Health.READ MORE
What Makes Good Brain Proteins Turn Bad?News
The protein FUS is implicated in two neurodegenerative diseases: amyotrophic lateral sclerosis (ALS) and frontotemporal lobar degeneration (FTLD). Using a newly developed fruit fly model, researchers have zoomed in on the protein structure of FUS to gain more insight into how it causes neuronal toxicity and disease.