We've updated our Privacy Policy to make it clearer how we use your personal data.

We use cookies to provide you with a better experience. You can read our Cookie Policy here.

Advertisement
Discover the Mysteries of the Brain with Applied Biosystems iTRAQ Reagents
News

Discover the Mysteries of the Brain with Applied Biosystems iTRAQ Reagents

Discover the Mysteries of the Brain with Applied Biosystems iTRAQ Reagents
News

Discover the Mysteries of the Brain with Applied Biosystems iTRAQ Reagents

Read time:
 

Want a FREE PDF version of This News Story?

Complete the form below and we will email you a PDF version of "Discover the Mysteries of the Brain with Applied Biosystems iTRAQ Reagents"

First Name*
Last Name*
Email Address*
Country*
Company Type*
Job Function*
Would you like to receive further email communication from Technology Networks?

Technology Networks Ltd. needs the contact information you provide to us to contact you about our products and services. You may unsubscribe from these communications at any time. For information on how to unsubscribe, as well as our privacy practices and commitment to protecting your privacy, check out our Privacy Policy

Researchers at the Centre for Neurogenomics and Cognitive Research, Vrije Universiteit, in The Netherlands, have announced that they are using Applied Biosystems iTRAQ reagent kits to analyse proteins in brain synapses using tandem mass spectrometry (MS/MS) on the Applied Biosystems 4700 Proteomics Analyzer.

The team are characterising the dynamic changes taking place in the synapse during the changing conditions involved in memory creation, learning and behaviour.

Dr Ka Wan Li, Assistant Professor, explained, "There are about 2000 different proteins in the synapse and they play major roles in brain function and plasticity."

"Unfortunately, many of them are membrane proteins, which cannot be easily separated on 2D gels. Instead we solubilise, digest and label them with iTRAQ reagents, then analyse them on the 4700 Proteomics Analyzer."

"The iTRAQ reagents label all the peptides in four different samples, which means we do 10,000 or more MS/MS mass spectra per experiment."

"Using the iTRAQ reagents with the 4700 Proteomics Analyzer has been a turning point in our research because it means we can perform quantitative analyses of the synapse proteome on a routine basis."

Advertisement