Flexibility of a Eukaryotic Lipidome - Insights from Yeast Lipidomics
News Apr 30, 2012
Mass spectrometry-based shotgun lipidomics has enabled the quantitative and comprehensive assessment of cellular lipid compositions. The yeast Saccharomyces cerevisiae has proven to be a particularly valuable experimental system for studying lipid-related cellular processes. Here, by applying our shotgun lipidomics platform, we investigated the influence of a variety of commonly used growth conditions on the yeast lipidome, including glycerophospholipids, triglycerides, ergosterol as well as complex sphingolipids. This extensive dataset allowed for a quantitative description of the intrinsic flexibility of a eukaryotic lipidome, thereby providing new insights into the adjustments of lipid biosynthetic pathways. In addition, we established a baseline for future lipidomic experiments in yeast. Finally, flexibility of lipidomic features is proposed as a new parameter for the description of the physiological state of an organism.
The article is published online in PLoS ONE and is free to access.
The research labs of the associate professor Dr. Thomas Becker and Prof. Dr. Nikolaus Pfanner from the Institute for Biochemistry and Molecular Biology at the University of Freiburg discovered a function of the metabolite channel of the mitochondrial outer membrane in protein transport.READ MORE