In Cell Molecular Sieve From Protein Crystal
Credit: Tokyo Institute of Technology
Scientists at Tokyo Institute of Technology, RIKEN, and Kyoto Institute of Technology have applied rational crystal design to create protein crystals with extended porous network to accumulate exogenous molecules inside living cells. This work lays a foundation for engineering of stable self-assembling crystalline porous materials which can concentrate and preserve bioactive substances in various cell types.
In nature, proteins are assembled into sophisticated and highly ordered structures, which enable them to execute numerous functions supporting different forms of life. The exquisite design of natural proteins prompted scientists to exploit it in synthetic biology to engineer molecules that can self-assemble into nanoparticles with desired structure and that may be used for various purposes such as gas storage, enzyme catalysis, intracellular drug delivery, etc.
Cytoplasmic polyhedrosis viruses (cypoviruses) infecting insects are embedded in protein crystals called polyhedra which shield the virus from damage. The structure of polyhedra crystals (PhCs) suggests that they can serve as robust containers which can incorporate and protect foreign molecules from degradation, ensuring their compositional and functional stability.
Extreme stability of polyhedra under harsh conditions is provided by dense packing of polyhedrin monomers in crystals with solvent channels of very low porosity, which, however, limits the incorporation of foreign particles. Research group led by Satoshi Abe and Takafumi Ueno at Tokyo Institute of Technology hypothesized that if a porous framework inside PhCs is extended without compromising crystal stability, PhCs can be used for accumulation and storage of exogenous molecules in living cells. As in natural PhCs, polyhedrin monomers form a trimer, the scientists assumed that if amino acid residues at the contact interface of each trimer are deleted, the porosity of the resulting crystals would be increased.
To achieve this goal, they genetically engineered polyhedrin monomers, which were then expressed and self-assembled in Spodoptera frugiperda IPLB-Sf21AE, the larva of an armyworm moth, infected with baculovirus. The mutant PhCs maintained crystal lattice of the wild-type PhC but had significantly extended porosity due to the deletion of amino acid residues with the rearrangement of intra- and intermolecular hydrogen bonds.
As a result, the engineered crystals could adsorb 2-4 times more exogenous molecules (fluorescent dyes) compared to the wild type PhC, with up to 5,000-fold condensation of the dyes from the 10 uM solution. As a next step, the scientists examined the performance of the mutant crystals in living insect cells. PhCs showed high stability in the intracellular environment. Most importantly, the mutant crystals could accumulate and retain the dyes in live cells, while the natural crystals could not.
Rationale crystal design used by scientists at Tokyo Institute of Technology provides a powerful tool for structural manipulation of self-assembled protein crystals to obtain porous nanomaterials with regulated adsorption properties. The engineered porous PhCs can be used as protein containers for in vivo crystal structure analysis of the cellular molecules and bioorthogonal chemistry in various types of living cells.
Since tiny crystals with only a few microns size were obtained, the structure analyses were performed at beamlines BL32XU and BL41XU at SPring-8, a large synchrotron radiation facility which delivers the most powerful synchrotron radiation. The high-resolution structures were rapidly analyzed with the help of an automated data collection system developed in RIKEN.
Abe, S., Tabe, H., Ijiri, H., Yamashita, K., Hirata, K., Atsumi, K., Ueno, T. (2017). Crystal engineering of self-assembled porous protein materials in living cells. ACS Nano. doi:10.1021/acsnano.6b06099
This article has been republished from materials provided by Tokyo Institute of Technology . Note: material may have been edited for length and content. For further information, please contact the cited source.
Bioinformatics to Help Understand Intrinsically Disordered ProteinsNews
Over the last several decades, scientists have sequenced 85 million unique proteins, structured and unstructured alike, but still don’t know what the vast majority of these proteins do.READ MORE
Ingestible Drug-Delivery Materials May Improve Patient Treatment ComplianceNews
Hydrogel-based capsules could expand and reside in the GI tract for days, slowly releasing medication.READ MORE
Protein Discovery Could Lead to Better Diagnosis of StressNews
Researchers have found a protein that is present in people while they are in stressful situations. The discovery could lead to identifying new ways to predict, diagnose and treat stress.READ MORE
Comments | 0 ADD COMMENT
6th Edition of International Conference on Pharmacognosy and Medicinal Plants
Apr 16 - Apr 17, 2018
10th Edition of International Conference on Structural Biology 2018
Mar 15 - Mar 17, 2018
International Conference and Exhibition on Nanomedicine and Nanotechnology
Nov 23 - Nov 24, 2017