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Microglia Linked to Brain Atrophy in Progressive MS

Microglia Linked to Brain Atrophy in Progressive MS content piece image
Rat cerebellar molecular layer stained with IBA-1 antibody which reveals microglial cells, in red. Credit: GerryShaw [CC BY-SA 4.0 (https://creativecommons.org/licenses/by-sa/4.0)]
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Secondary progressive multiple sclerosis (MS) is a relentless disease. Over time, patients experience worsening physical, mental and mood-related symptoms. And yet, even as their symptoms progress, the brain white matter lesions found on a patient’s MRI scans often remain unchanged. Suspecting that changes in the grey matter regions of the brain may be playing a critical role in the disease’s progression, investigators from Brigham and Women’s Hospital conducted an initial study assessing the differences in the activity of microglial cells in the grey matter regions of healthy volunteers versus those with MS. Using a novel tracer molecule known as [F-18]PBR06 and Positron Emission Tomography (PET) imaging, the team detected widespread and abnormal activation of microglia in MS patients and a link to brain atrophy, physical disability, and progressive MS. The team’s findings are published in the July 2019 issue of Neurology: Neuroimmunology & Neuroinflammation, an official journal of the American Academy of Neurology.

“There’s more to multiple sclerosis than white matter lesions,” said corresponding author Tarun Singhal, MD, a neurologist at the Ann Romney Center for Neurologic Diseases at the Brigham. “There’s evidence of inflammation in the brain’s grey matter, not just the white matter. Here we have a technique to detect it and a path to develop this technique for use in the clinic in looking for early signs of progression and the effects of treatments.”

Currently, there are several disease-modifying drugs on the market to help patients with relapsing remitting MS experience fewer and less severe relapses, but few drugs exist for patients with secondary progressive MS. Investigations to develop new drugs are underway, but many questions remain about the underlying biology of the disease and how it progresses.

The new study leverages the novel radioisotope, [F-18]PBR06, a tracer that targets a specific protein (TSPO) found in activated microglia, key immune cells found in the brain. Many other research projects use C-11, an isotope with a much shorter half-life. But, unlike C-11, the F-18 tracer has a significantly longer half-life and a higher potential to be used in the clinic.

For the pilot study, investigators evaluated results for 12 patients with MS — seven with relapsing remitting MS and five with secondary progressive MS — and compared it with healthy controls using the F-18 tracer. They found more grey matter microglial activation in the MS patients as compared to healthy controls, particularly in hippocampus, parahippocampus, cingulate gyrus and amygdala regions of the brain. These regions of the brain are known to influence critical processes, including emotion, memory and cognition, all of which may be affected in MS patients. Brain structures in the deep grey matter, particularly the thalamus, showed higher microglial activation in secondary progressive MS than in the relapsing remitting MS patients and healthy controls. This correlated significantly with physical disability and brain atrophy.

The authors note that the pilot study is small and its findings will require additional confirmation in larger studies with a longitudinal design, but it offers the first assessment of [F-18]PBR06 PET for grey matter changes in MS, demonstrating the potential value of this technique.

“Unless we can measure the progress of a disease accurately, our ability to treat that disease remains limited,” said Singhal. “When a patient tells us that their symptoms are worsening, we want to have a technology that can reflect that, or better yet, predict the progression before it is clinically obvious. This technique may have the potential to do that and give us critical insights into neurodegeneration and its relationship with neuroinflammation.”

Other authors of this paper include Kelsey O’Connor, Shipra Dubey, Hong Pan, Renxin Chu, Shelley Hurwitz, Steven Cicero, Shahamat Tauhid, David Silbersweig, Emily Stern, Marie Kijewski, Marcelo DiCarli, Howard L Weiner, and Rohit Bakshi.

Funding for this work was provided by Nancy Davis Foundation’s “Race to Erase MS” program, Ann Romney Center for Neurologic Diseases, Harvard NeuroDiscovery Center, and Water Cove Charitable Foundation. Co-authors have received grant support from Spectrum Dynamics, Merck-Serono and Sanofi-Genzyme, Verily Life, EMD Serono, and Sanofi-Genzyme, and consulting fees from GE, Sanofi, Biogen, Tiziana, Novartis, Merck-Serono, Teva, Bayer, Celgene, EMD Serono, Genentech, Guerbet, Sanofi-Genzyme, and Shire.

Reference: Singhal, T et al. “Grey Matter Microglial Activation in Relapsing versus Progressive Multiple Sclerosis: An Initial Experience using [F-18]PBR06-PET” Neurology: Neuroimmunology & Neuroinflammation. DOI: 10.1212/NXI.0000000000000587

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