Biotech Support Group reports on a recent research article describing the simplicity and efficiency of their hemoglobin depletion technology for enriching the red cell sub-proteome, from human red blood cells. In brief, using proteomics-based evaluation of red blood cells (RBC), the authors identified differentially abundant proteins associated with Obstructive Sleep Apnea Syndrome (OSA).
Proteome variations between various time points were assessed. The article states “RBC cytoplasmic fraction depleted of hemoglobin, using Hemovoid™ system, were analyzed by two-dimensional fluorescence difference gel electrophoresis (2D-DIGE), the 2D image software-based analyzed and relevant differentially abundant proteins identified by mass spectrometry (MS)”. The authors conclude that 31 protein spots were differentially abundant, corresponding to 21 unique proteins possibly due to the existence of posttranslational modifications.
“This article illustrates the importance of removing hemoglobin in order to perform proteomic analysis of red cells. The ability to enrich for the underlying sub-proteome which would normally mask many proteins from red cells is a unique feature of HemoVoid™. Many differentially regulated proteins were identified, demonstrating its usefulness in proteomic investigations” states Swapan Roy, Ph.D., President and Founder of Biotech Support Group.
Feliciano, A., Vaz, F., Valentim-Coelho, C., Torres, V. M., Silva, R., Prosinecki, V., Penque, D. (2017). Evening and morning alterations in obstructive sleep Apnea red blood cell proteome. Data in Brief, 11, 103–110. doi:10.1016/j.dib.2017.01.005
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