New Article Shows Hemoglobin Depletion Technology for the Sub-Proteome
Biotech Support Group reports on a recent research article describing the simplicity and efficiency of their hemoglobin depletion technology for enriching the red cell sub-proteome, from human red blood cells. In brief, using proteomics-based evaluation of red blood cells (RBC), the authors identified differentially abundant proteins associated with Obstructive Sleep Apnea Syndrome (OSA).
Proteome variations between various time points were assessed. The article states “RBC cytoplasmic fraction depleted of hemoglobin, using Hemovoid™ system, were analyzed by two-dimensional fluorescence difference gel electrophoresis (2D-DIGE), the 2D image software-based analyzed and relevant differentially abundant proteins identified by mass spectrometry (MS)”. The authors conclude that 31 protein spots were differentially abundant, corresponding to 21 unique proteins possibly due to the existence of posttranslational modifications.
“This article illustrates the importance of removing hemoglobin in order to perform proteomic analysis of red cells. The ability to enrich for the underlying sub-proteome which would normally mask many proteins from red cells is a unique feature of HemoVoid™. Many differentially regulated proteins were identified, demonstrating its usefulness in proteomic investigations” states Swapan Roy, Ph.D., President and Founder of Biotech Support Group.
Feliciano, A., Vaz, F., Valentim-Coelho, C., Torres, V. M., Silva, R., Prosinecki, V., Penque, D. (2017). Evening and morning alterations in obstructive sleep Apnea red blood cell proteome. Data in Brief, 11, 103–110. doi:10.1016/j.dib.2017.01.005
This article has been republished from materials provided by Biotech Support Group. Note: material may have been edited for length and content. For further information, please contact the cited source.
Cancer Cells’ Energy Source Blocked by Natural CompoundNews
Researchers have not only untangled an unusual wiring system that cancer cells use for carbohydrate metabolism, but also identified a natural compound that appears to selectively shut down this system in laboratory studies.READ MORE
APC Protein Deletion Disrupts Cell Signalling and Could Cause AutismNews
Researchers show deletion of the protein APC in progenitor cells leads to massive disruption of brain development and a signaling cascade previously linked to genes associated with autism.READ MORE
SCIEX Receive Science and Technology Award at HUPO 2017News
Two researchers from SCIEX received the HUPO 2017 award at the 16th Human Proteome Organization World Congress in Dublin, Ireland.READ MORE