Protein Central to Immune Response Against Tuberculosis Bacteria Identified
News Jan 12, 2017 | Original Story From UT Southwestern Medical Center
UT Southwestern Medical Center researchers have identified a protein that is central to the immune system’s ability to recognize and destroy the bacterium responsible for the global tuberculosis (TB) epidemic. The new finding could someday lead to the development of immunity-based therapies to treat tuberculosisby, strengthening this immune pathway, said Dr. Michael Shiloh, Assistant Professor of Internal Medicine and Microbiology.
According to the World Health Organization, TB is a top infectious disease killer worldwide and is estimated to have infected 9.5 million people and caused 1.5 million deaths in 2014. That year, tuberculosis surpassed HIV as the world’s most lethal infection.
“The protein Smurf1 functions in specialized white blood cells called macrophages in both mice and humans, thereby suggesting a conserved evolutionary pathway,” said Dr. Shiloh, co-senior author of the study along with Dr. Beth Levine, Director of the University’s Center for Autophagy Research.
In 2011, UT Southwestern researchers in Dr. Levine’s laboratory identified the protein Smurf1 as important for the elimination of viruses and damaged mitochondria from cells via a cellular housekeeping process called autophagy. That result led to the current study, a collaboration between the Shiloh and Levine laboratories to determine if Smurf1 plays a similar role in the autophagy of bacteria like M. tuberculosis inside cells.
In addition to recycling components of the cell to provide nutrients during starvation and acting as quality control for the organelles and proteins inside cells, autophagy helps eliminate pathogens such as viruses, parasites, and bacteria that get inside the cell. During antibacterial autophagy, the bacteria get tagged with the protein ubiquitin, marking them for destruction by an organelle called the lysosome. The role of Smurf1, one of hundreds of E3 ubiquitin ligases in mammals, was unknown in this process.
In this study, the researchers found that macrophages from mice lacking Smurf1 were unable to attach the death-tagging protein ubiquitin to intracellular bacteria, resulting in a failure of the autophagy pathway and runaway growth of the bacteria inside the cells. When infected with TB, mice lacking Smurf1 had higher bacterial loads, increased lung inflammation, and accelerated mortality compared to mice with normal Smurf1 activity, Dr. Shiloh said.
The researchers next showed that the Smurf1 gene controls M. tuberculosis growth in human macrophages, and that the Smurf1 protein was found in association with bacteria in the lungs of patients with tuberculosis infections.
“Even though humans mount a defense against M. tuberculosis that can contain its growth, in general that defense is insufficient to kill the bacteria,” Dr. Shiloh explained. “Finding ways to harness or enhance the autophagy pathway and Smurf1 could lead to new strategies to kill intracellular bacteria like those that cause TB,” he added.
Franco, L. H., Nair, V. R., Scharn, C. R., Xavier, R. J., Torrealba, J. R., Shiloh, M. U., & Levine, B. (2017). The Ubiquitin Ligase Smurf1 functions in selective Autophagy of Mycobacterium tuberculosis and Anti-tuberculous host defense. Cell Host & Microbe, 21(1), 59–72. doi:10.1016/j.chom.2016.11.002
Please note: The content above may have been edited to ensure it is in keeping with Technology Networks’ style and length guidelines.
SMi’s 5th Annual Molecular Diagnostics Conference 2018 Agenda ReleasedNews
SMi proudly presents its 5th annual conference on Molecular Diagnostics, taking place at the Holiday Inn Kensington Forum, London, UK on 9th and 10th July 2018, with a half-day post-conference workshops on 11th July 2018.READ MORE
Low Temperatures Turn Stem Cells into Calorie-Burning FatNews
The secret to healthy eating may not be in freezer food, but low temperatures can help turn stem cells into brown fat, a type of fat which helps burn calories. A new study could turn this knowledge into weight-loss treatments.READ MORE
Macrophage's Role in Maintaining Tattoos Could Hold Key to RemovalNews
Researchers have discovered that, though a tattoo may be forever, the skin cells that carry the tattoo pigment are not. Instead, the cells can pass on the pigment to new cells when they die. The study suggests ways to improve the ability of laser surgery to remove unwanted tattoos.READ MORE
Comments | 0 ADD COMMENT
International Conference on Neuroimmunology, Neurological disorders and Neurogenetics
Sep 26 - Sep 27, 2018