Synthetic Protein Eases Arthritis Symptoms in Mice
News Oct 04, 2005
Scientists from the National Institute of Allergy and Infectious Disease (NIAID) have announced that a lab-made version of a human protein alleviates symptoms of both acute and chronic arthritis in mice and could be the basis for an arthritis drug for people.
The protein prevents the assembly of a cell surface receptor, thus blocking transmission of chemical signals that lead to arthritis symptoms.
“This study opens a new research avenue to better understand and, perhaps, to treat rheumatoid arthritis, a condition that causes suffering in more than two million Americans,” says NIAID Director Anthony S. Fauci, M.D.
Investigators from NIAID's Laboratory of Immunology, led by Michael Lenardo, M.D., published their findings in the October issue of “Nature Medicine”, now available online.
The idea that the protein, called pre-ligand assembly domain protein or PLAD, might play a role in thwarting the joint inflammation characteristic of rheumatoid arthritis, grew out of their research on a very rare autoimmune disease called autoimmune lymphoproliferative syndrome (ALPS).
Previously, Dr. Lenardo and his colleagues showed that in ALPS a form of PLAD blocks a cell surface receptor and prevents a needed chemical signaling pathway from functioning correctly.
In ALPS, the signal pathway interrupted by PLAD leads to disease symptoms. But, the scientists reasoned, PLAD might also be able to block a related cell surface receptor - one involved in passing signals that lead to inflammation.
In theory, inhibiting this pathway might benefit people with rheumatoid arthritis, who suffer from excessive inflammation.
A key promoter of inflammation is a chemical called tumor necrosis factor alpha (TNF-alpha).
TNF-alpha starts a chemical chain reaction leading to inflammation by binding to two cell surface receptors, TNFR-1 and TNFR-2.
Naturally occurring PLAD helps both forms of TNFR assemble and prepare to receive TNF-alpha.
Synthetic PLAD, the scientists hypothesized, would bind to its natural counterpart and prevent it from performing its usual task.
“We found that P60 PLAD protein powerfully inhibited the symptoms of TNF-alpha-induced arthritis,” says Dr. Lenardo.
P60 PLAD also lessened the effects of arthritis induced by other means. Moreover, he adds, P60 PLAD appeared to inhibit disease symptoms in mice with established as well as acute arthritis.
“We're very hopeful that this could be good news for arthritis sufferers,” says Dr. Lenardo.
In particular, the researchers are intrigued by P60 PLAD's apparent specificity: it seems to block the binding of TNF-alpha to TNFR-1, while allowing TNFR-2 to continue to function.
This is important, notes Dr. Lenardo, because it may represent an advantage over some currently used arthritis drugs, which directly block TNF-alpha by binding to both TNFRs and thereby inhibit beneficial actions mediated by TNFR-2.