TAP Biosystems Partners with Life Technologies on Protein Expression Platform
News Jan 15, 2013
The partnership will combine Life Technologies’ Expi293™ Expression System for transient transfection with TAP’s ambr™ advanced micro bioreactor to enable automated high throughput small scale protein expression. The increased culture consistency and productivity provided by both the Expi293 and ambr systems will offer an exciting new platform for both small volume protein expression screens and optimisation of transient expression conditions.
The ambr system uses realistic scale down bioreactors where culture conditions such as pH and DO are tightly controlled in 48 micro bioreactors (10-15 mL) in parallel, so that protein quality produced by the Expi293F cells cultured in ambr is highly consistent. The protein generated can be used with confidence in vector optimisation studies, as well as high throughput, small scale functional protein production and protein purification studies.
Dr Sanjay Vasu, Staff Scientist at Life Technologies explained: “Currently with Expi293F cells we’re seeing yields of up to 1g/L, exceeding the expression levels of even bacterial systems. The benefit of this is that scientists will have sufficient protein for analytical studies. And since this is a mammalian system, mammalian proteins are more likely to be properly folded and biologically active. We are pleased to be collaborating with TAP because using the ambr system’s automated bioprocessing capabilities; we can leverage both our platforms to provide high yields in a high throughput format for lower costs, which will represent a real breakthrough for many protein screeners.”
Dr Barney Zoro, ambr Product Manager at TAP Biosystems added: “Most high throughput protein expression is performed manually in 30mL shake flask cultures or in shaking plates, where there isn’t consistent pH and DO control. Manually intensive shake flask workflows are costly to operate, can produce variable results for productivity and protein quality, and often show poor scalability to larger bioreactor cultures. Since the Expi293 Expression System can produce milligram to gram quantities of protein with consistent quality, it is ideal to amplify this with the consistent automation and proven scalability of the ambr system. This will enable scientists to rapidly automate the generation of enough protein for analysis from 48 parallel cultures, thus saving laboratory staff time, increasing laboratory capacity and significantly reducing the cost per gram of protein produced. This powerful technology combination has the potential to revolutionise high throughput protein expression programmes and we’re delighted to be part of it.”
Synthetic DNA Shuffling Enzyme Outpaces Natural CounterpartNews
A new synthetic enzyme, crafted from DNA rather than protein, flips lipid molecules within the cell membrane, triggering a signal pathway that could be harnessed to induce cell death in cancer cells. Researchers say their lipid-scrambling DNA enzyme is the first in its class to outperform naturally occurring enzymes – and does so by three orders of magnitudeREAD MORE
Eating Activates Calorie-Burning FatNews
The importance of the human brown adipose tissue (BAT) has become clearer during the past ten years. Coldness is one of the most effective activators of the BAT metabolic function but, in rodents, eating has also been shown to activate BAT. The debate on whether eating has the same effect on humans has lasted for decades. Now, the researchers at Turku PET Centre have proven that having a meal increases oxygen consumption in human BAT to the same extent as coldness.READ MORE