The National Center for Genome Resources Studies Childhood Diseases Using febit’s DNA Capture Method
News Aug 11, 2009
febit’s HybSelect™ technology for sequence capture enables targeted Next-Generation Sequencing, furthers development of genetic tests for childhood diseases.
The National Center for Genome Resources (NCGR) has selected febit’s DNA-capture method, HybSelect™, to re-sequence the exons of genes involved in over 400 of the most devastating childhood diseases with the ultimate goal of developing genetic tests to detect carrier status for recessive genetic diseases in prospective parents.
In this collaboration the NCGR will bring its experience in comprehensive Illumina sequencing studies to febit’s automated HybSelect technology for targeted re-sequencing to develop and test highly parallel analysis. This will enhance the multiplexing capability of HybSelect to perform surveys in large-cohort studies.
NCGR scientists searched for a technology that would allow them to capture the exons of interest for re-sequencing. febit’s automated HybSelect technology for targeted, hybridization-based DNA enrichment supports deep sequencing studies resulting in high coverage results. The Geniom RT Analyzerâ provides the easy-to-use and highly automated capturing of the desired DNA fragments for further Next-Generation Sequencing (NGS).
Stephen Kingsmore, CEO of NCGR, stated: “We are impressed by the unique flexibility and scalability of febit’s HybSelect technology and with the ease of integration in production sequencing with Illumina Genome Analyzers. In partnership with the Beyond Batten Disease Foundation, we aim to combine these technologies to offer highly cost effective screening for rare childhood diseases.”
Previous work by the International Multiple Sclerosis Genetics Consortium (IMSGC) has identified 233 genetic risk variants. However, these only account for about 20% of overall disease risk, with the remaining genetic culprits proving elusive. A new study has tracked down four of these hard-to-find genes.READ MORE