Neuronal Mislocalization of Mutant DHHC9 in X-Linked Intellectual DIsability
DHHC9 is a protein acyltransferase (PAT) that adds a palmitate to specific protein substrates. In the neuron, palmitoylation of various proteins by DHHC9 facilitates proper neuronal function. Recently, a DHHC9 nonsense mutation (R298*), resulting in a truncation of the last 67 amino acids of the protein, has been associated with X-Linked Intellectual Disability (XLID). Here, we compare neuronal localization of the wild-type (WT) and R298* mutant DHHC9.
Cross-disciplinary Optimization of Nano-Drug Delivery to Ovarian Carcinoma and Glioma Cells
This study reports on the quantitative analysis of the diffusion and localization of a targeted drug delivery system (DDS) consisting of fluorescent labeled 0.01 µM paclitaxel-BODIPY 564/570 encapsulated in non-ionic surfactant vesicles embedded in a thermosensitive cross-linked chitosan hydrogel.
Hexachlorophene reduces Tau aggregation and potential therapeutic agent for treatment of Alzheimer’s disease
An increase in phosphorylation of tau (hyper phosphorylation) leads to aggregation of the protein resulting in neurofibrillary tangles, a pathological hallmark associated with Alzheimer’s disease (AD). The identification of pharmacological agents that can help decrease levels of phosphorylated tau would be advantageous. Recent work in our laboratory has found that the molecule hexachlorophene can regulate levels of tau in cellular models.
Astaxanthin Attenuates Neurotoxicity in a Mouse Model of Parkinson’s disease
Both oxidative stress and inflammation are involved in the progression of many neurodegenerative diseases, therefore, we examined the potential for AXT to reduced neurotoxicity in a toxic model of PD in mice. Here, we show that administration of algae derived AXT mitigated MPTP induced dopamine cell death.
Discrepancy between TRPA1 Activation by Reversible and Irreversible Electrophiles Suggests Involvement of Cytosolic Cofactors Other Than Polyphosphates
Electrophiles can bind through reversible or irreversible reactions based on the structure of the compound. Here, patch clamp techniques and calcium imaging have been used to characterize human TRPA1 (hTRPA1) channel activity (expressed in HEK293 cells) in the presence of reversible and irreversible electrophiles
Acoustic Startle Response as a Prognostic Tool for Traumatic Brain Injury
The Acoustic Startle Reflex (ASR) is a brain-stem mediated, tri-synaptic response to acoustic stimuli involving involuntary contraction of major muscle groups. Previous studies have demonstrated that this response is suppressed following the fluid-percussion model of traumatic brain injury (TBI). The possibility exists that the suppression of this response could be exploited for prognostic purposes.
Examining the Role of Arginase1 Overexpression in an Animal Model of Tauopathy
Because of the possible beneficial role of polyamines, metabolism of arginine by arginase is of interest. Recently, our lab has recently shown that arginase1 overexpression has the ability to reduce phosphorylated tau and neurofibrillary tangles in rTg4510 and PS19 tau transgenic mice. Based on these data, we utilized the transgenic tetO MAPT*P301L mouse model to examine a potential therapeutic gene of interest, arginase1, in an animal model of tauopathy.
Neuroprotection by T-Lymphocytes and Stem Cells After Ischemic Stroke
In this study, regulatory T-cells and BMSCs were shown to be neuroprotective following ischemic treatment of primary rat neurons.
Stress-induced nucleocytoplasmic shuttling of TDP-43 is controlled by eIF-5A hypusination
In this experiment, we sought to determine the function of hypusinated eIF5a in relation to TDP-43 pathology in nuclear and cytoplasmic compartments under cellular stress.
Role of TDP-43 in activation of the brain inflammatory response
Profiling of inflammatory cytokines, receptor and kinases in WT and TDP-43 overexpressing mice.