Acoustic Startle Response as a Prognostic Tool for Traumatic Brain Injury
Poster Feb 13, 2017
M. Grant Liska, Vivian A. Guedes, Connor Stonesifer, Lindsey Gelineau, Marci Crowley, David J. Eve, Cesar V. Borlongan*
Traumatic Brain Injury (TBI) is a leading cause of death and disability in military and civilian populations. TBI can occur from a number of insults - such as blunt force, blast impact, or penetrating wounds - all associated with acute cognitive and sensory symptoms. The Acoustic Startle Reflex (ASR) is a brain-stem mediated, tri-synaptic response to acoustic stimuli involving involuntary contraction of major muscle groups. Previous studies have demonstrated that this response is suppressed following the fluid-percussion model of traumatic brain injury (TBI). The possibility exists that the suppression of this response could be exploited for prognostic purposes.
In order to generate a robust protocol for MEA recording on hiPSC- derived neurons, we evaluated several conditions, which could affect culture performance (1.neuron seeding density; 2.seeding medium; 3.astrocyt eco-culture). These conditions were evaluated with BrainXell’s hiPSC-derived spinal motor neurons, cortical glutamatergic neurons and mixed cortical neurons.READ MORE