C1 Complement mediates human cord blood serum derived APP α-secretase cleavage activity in vitro
Poster May 08, 2018
Anran Fan, Catherine Wang, Ahsan Habib, Darrell Sawmiller, Huayan Hou,1Manasa Kanithi, Dan Zi, Zhixu He, Paul R. Sanberg and Jun Tan.
Alzheimer’s disease (AD) is the leading cause of dementia in the elderly. In healthy individual amyloid precursor protein (APP) is cleaved by α-secretase generating sAPPα. However, in the neurodegenerative environment of AD patients, Aß peptides of either 40 or 42 residues are generated by increased beta and gamma secretase activity. Human umbilical cord blood cells (UCBC) have proven useful as potential immunomodulatory therapies in various models of neurodegenerative diseases. Our study investigated the impact of UCBS on modulation of sAPPα production. Heat-activated UCBS has significantly promoted sAPPα production indicating presence of heat sensitive α-secretase in CBS. Using LC-MS/MS, We identified the subunits of C1 complex (C1q, C1r and C1s) and α-2-macroglobulin showed significantly greater levels in αCBSF compared with AgBSF. Specifically, C1 markedly increased sAPPα and αCTF production, whereas C1q alone only minimally increased and C3 did not increase sAPPα production in the absence of sera. Furthermore, C1q markedly increased sAPPα and αCTF, while decreasing Aß, in CHO/APPwt cells cultured in the presence of whole sera. These results confirm that APP α-secretase activity in human blood serum is mediated by C1 opening a potential modality of therapeutic for the future of AD.
Identifying Non-Intentionally Added Substances (NIAS) in Food Contact MaterialsPoster
Concern about the safety of food products has increased dramatically with intentionally and non-intentionally added substances (NIAS) used in packaging being of particular interest. APGC and high resolution MS together with a workflow driven process was used to identify unknown compounds in packaging.READ MORE
Human exposure to cyanotoxins: Exploring in vitro detoxification using atmospheric cold plasma treatment to protect human healthPoster
Recently, there have been increases in freshwater harmful algal blooms. There is therefore a need to try and effectively detoxify drinking water contaminated with cyanotoxins to safeguard human health by use of new and novel techniques.READ MORE
Psychiatric Risk Gene Cacna1c and Early Life Stress: Potential Gene-Environment interactions?Poster
Early life stress (ELS) is highly associated with development of psychopathology
and mood disorders in adulthood. Genetic studies have identified variation in the gene calcium voltage-gated channel subunit alpha1C (CACNA1C) to increase risk for several psychiatric disorders. This poster assessed the expression of Cacna1c following prepubertal stress.