CiPA Phase 2 Study: validation of an automated microelectrode array (MEA) assay of hiPSC-derived cardiomyocyte electrophysiology for cardiac safety evaluation
Poster Mar 10, 2017
DC Millard, AM Nicolini, SA Chvatal, HB Hayes, M Clements, JD Ross
The Comprehensive in vitro Proarrhythmia Assay (CiPA), led by the US Food and Drug Administration, aims to reduce unwarranted drug attrition by improving preclinical cardiac safety evaluation. A key component of the proposed assay utilizes human stem cell derived cardiomyocytes (hSC-CMs) and microelectrode array (MEA) technology to evaluate the proarrhythmic risk of candidate compounds . An initial pilot study with 8 blinded compounds demonstrated reproducible results across 10 studies. The CiPA Phase 2 study with 28 blinded compounds was established to evaluate the ability of the CM-MEA assay to assess proarrhythmic risk. The increased scale of the CiPA Phase 2 study was used as a case study in the development and validation of an automated CM-MEA assay for cardiac safety evaluation.
The iCell Cardiomyocyte2 and Maestro APEX automated MEA workstation were used to evaluate 28 compounds of high, intermediate, and low proarrhythmic risk in a blinded study. The amplitude and field potential duration (FPD) were extracted from the raw field potential signal for each replicate and compound, providing information on the depolarization and repolarization of the cardiomyocyte monolayers, respectively. Additional beating characteristics, such as the beating rate, variability in beat rate, and occurrence of EADs were also included in the analysis. Corrected field potential duration (FPDc) was computed using the Fridericia correction.
These results support the use of hSC-CM and MEA technology for preclinical assessment of proarrhythmic risk within the proposed CiPA paradigm, and, more generally, demonstrate that automation of the CM-MEA assay can achieve high reliability and throughput for cardiac risk assessment in vitro.
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Early life stress (ELS) is highly associated with development of psychopathology
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Regulatory T-Cells (Tregs) Within Bone Marrow-Derived Stem Cells (BMSCs) Actively Confer Immunomodulatory and Neuroprotective Effects Against StrokePoster
We found a distinct subpopulation of Tregs within BMSCs. Tregs and BMSCs in co-culture conferred neuroprotection that varied in a dose-dependent manner. Tregs minimized stem cell production of IL-6, a pro-inflammatory cytokine, and inhibited BMSC secretion of FGF-beta, a cytokine related to BMSC proliferation and differentiation. The ratio of Tregs found natively in BMSCs is optimally adapted to provide the maximum neuroprotective benefit of stem cell treatment after ischemic stroke.READ MORE
5th edition of the International Conference Clinical Oncology and Molecular Diagnostics
Jun 17 - Jun 18, 2019