Designing a Model to Explore Tau's Unfolded Protein Response
Poster Mar 07, 2018
Lauren Gould, Roy Blackburn, Laura J. Blair
It is known that the accumulation of the protein tau leads to neuronal loss that cause the mental deficits associated with AD. While tau’s relationship with increased neurotoxicity in the brain is known, the method as to how tau triggers the activation of the unfolded protein response (UPR) is unclear and has not been highly explored.
The purpose of this research is to design a cell model in which ER stress caused by tau accumulation can be generated, and then investigated for changes in different ER stress-associated proteins. The data provided by this experiment will allow for a working in vitro model of tau UPR and its effects on ER stress that will allow enhanced understanding of how tau causes neuronal death in AD.
Knockout of microRNAs Using the CRISPR-Cas9 System with Paired Synthetic crRNAsPoster
We utilized paired synthetic crRNAs coupled with our synthetic tracrRNA in cells transduced with lentiviral Cas9 to perform a functional knockout on hsa-miR-221. This three-part system (crRNA, tracrRNA and Cas9) has demonstrated efficient gene editing when used with only one guide RNA, but the goal was to use two crRNAs to remove the entire stem-loop.READ MORE
A New Method for Analyzing MSe/All Ions Fragmentation in Xenobiotic Metabolism StudiesPoster
During early drug discovery, the study of metabolism plays an essential role in determining which drug candidates move forward into development and later stages. As an alternative to traditional Data Dependent Acquisition (DDA), the use of MSE/All Ions Fragmentation (AIF) has become common in metabolite identification workflows for the analysis of metabolic hot spots. Here we present a solution for analysis of MSE/AlF in metID studies.READ MORE
Extracorporeal shockwave therapy accelerates motor axon regeneration despite a phenotypically mismatched environmentPoster
A femoral nerve defect model was adapted for the evaluation of proregenerative effects of extracorporeal shockwave therapy (ESWT). Functional evaluation, histology and qRT-PCR data show differences between sensory and motor-derived nerve transplants and a pro-regenerative effect of ESWT. These data provide evidence for the clinical application of ESWT after autologous nerve transplantation as a novel non-invasive method.READ MORE