Proteomic Analysis of Cell Surface Proteins with Improved Specificity of Enrichment
Poster Dec 05, 2019
Betsy Benton, Sergei Snovida, Katherine Herting, Hongbin Zhu, John C. Rogers, and Barbara Kaboord
Cell surface proteins are a rich source of therapeutic targets for disease and play a major role in signal transduction, cell adhesion and ion transport. Cell surface biotinylation is a popular method for isolating such cell surface proteins, but the process is not without issues. However, alternative methods are being developed.
Download this poster to discover one such alternative and how this method:
- Improves selectivity and enrichment
- Reduces the level of contamination
- Is compatible with mass spectrometry
Multiplexing cell-based assays is possible using 3D culture models that are larger and more complex than monolayers
Real-time detection methods to measure live or dead cells provide much flexibility for multiplexing
All multiplexed assay combinations should be verified using appropriate controls for each 3D cell culture model.