Proteomics and Substrate Based MS Imaging of Xenobiotic Metabolising Enzymes in Ex Vivo Human Skin and a Human Living Skin Equivalent Model
Poster Nov 21, 2017
J. R. A. Newton, N. Couto, J. Barber, R. Bojar, J. Sidaway and M. Clench
Current animal-free approaches for assessing the metabolism of xenobiotics in skin are limited. The primary reason for this is that the levels and distribution of xenobiotic metabolising enzymes in skin are not well characterised. The overall aim of this project was to develop a combined in silico-in vitro system for predicting xenobiotic metabolism in human skin from experiments carried out on a living skin equivalent (LSE). In order to do this it was required to systematically quantify the level of xenobiotic metabolising enzymes in skin and in Labskin using proteomics.
In order to examine the distribution of enzymes detected, a novel substrate-based mass spectrometry imaging technique was proposed that would allow the activity of xenobiotic metabolising enzymes to be localised within the in vitro model. Ultimately the project will use these proteomic and imaging data to build a pharmacokinetic (PBPK) model of human skin xenobiotic metabolism and disposition to enable in vitro to in vivo extrapolation (IVIVE) of LSE based xenobiotic metabolism data. This integrated solution would then offer an animal-free alternative that will increase screening capacity and knowledge of xenobiotic skin metabolism.
Treatment Options for Chronic Parvovirus Viremia in Pediatric Heart Transplant Patients in a Tertiary Care CenterPoster
This abstract discusses three cases of pediatric heart transplant patients who suffered from parvovirus (B19) infection. Of these patients, two ( B & C) responded well to standard intravenous Ig therapy. Patient A however, did not respond to standard treatment and was begun on subcutaneous Ig, which effectively diminished his viral load. Thus, subcutaneous Ig infusions might serve as a second line treatment for transplant patients with parvovirus who do not respond well to the standard approach.READ MORE
T-helper cell phenotype expression in cutaneous lesions of angioimmunoblastic T-cell lymphomaPoster
Angioimmunoblastic T-cell lymphoma (AITL) is a common type of peripheral T-cell lymphoma. AITL can be missed until lymphadenopathy develops in patients initially presenting with skin lesions, as skin biopsy may lack conclusive findings. Our case highlights the extranodal presentation of AITL with cutaneous lesions displaying the TFH phenotype.READ MORE
Regulatory T-Cells (Tregs) Within Bone Marrow-Derived Stem Cells (BMSCs) Actively Confer Immunomodulatory and Neuroprotective Effects Against StrokePoster
We found a distinct subpopulation of Tregs within BMSCs. Tregs and BMSCs in co-culture conferred neuroprotection that varied in a dose-dependent manner. Tregs minimized stem cell production of IL-6, a pro-inflammatory cytokine, and inhibited BMSC secretion of FGF-beta, a cytokine related to BMSC proliferation and differentiation. The ratio of Tregs found natively in BMSCs is optimally adapted to provide the maximum neuroprotective benefit of stem cell treatment after ischemic stroke.READ MORE