454 Life Sciences Unveils New Bench Top Sequencer
Product News Nov 23, 2009
454 Life Sciences, a Roche Company, has announced at the Association of Molecular Pathology Annual Meeting in Kissimmee, Florida a series of developments expanding the company’s high-throughput sequencing portfolio.
Addressing the growing demand for next-generation sequencing data in everyday biological and clinical research, the company revealed the new GS Junior System, a bench top sequencing platform slated for release in 2010.
The GS Junior System, which is no bigger than a typical laser printer, has performance and features tuned to fit the needs of small to medium sized laboratories. The platform will launch with long-read GS Junior Titanium chemistry, offering 400 – 500 bp read lengths, and will be shipped with a desktop computer that is optimized for GS Junior Run processing and downstream data analysis.
“With the GS Junior System, we are extremely excited to make next-generation sequencing readily available to the individual researcher, just as the PC brought computing to the masses,” said Christopher McLeod, President and CEO of 454 Life Sciences. “This truly enabling technology is now affordable for nearly every research lab. At the same time, we continue to extend the performance of our flagship Genome Sequencer FLX System.”
The company announced plans to initiate an early access program for development of the next set of performance improvements to the Genome Sequencer FLX System, which will double the read length of the current GS FLX Titanium Kits and expect to contain reads up to 1,000 bp. The DOE Joint Genome Institute will be the first site to begin early access later this month and will use the long-read chemistries to sequence a variety of large, complex genomes. Additional early access partners are expected to be announced in the near future.
“The value of our longer read lengths, coupled with the latest advances in our assembler software, have been demonstrated by the successful conclusion of a number of recent de novo sequencing projects, such as the cod fish genome,” explained Michael Egholm, CTO and Vice President of R&D of 454 Life Sciences. “The Genome Sequencer FLX System provides a robust platform to sequence and assemble nearly any size genome quickly, without the need for supplementary Sanger data.”
The company’s final announcement at the event was the launch and immediate availability of several new kits, protocols and software updates for the Genome Sequencer FLX System. The new products enable two new major application areas to take advantage of the long 400-500 bp reads and increased density of the GS FLX Titanium series chemistry - transcriptome sequencing and amplicon (PCR product) sequencing.
Additional product updates highlight the company’s continued efforts to ease workflow, including a new Rapid Library Preparation Kit which takes around half of the time and requires significantly less input DNA, and a set of 12 Rapid Library Multiplex Identifier (MID) Adaptors for sample pooling.
Significant improvements to the complete suite of GS Data Analysis Software are evident in the new GS De Novo Assembler software, now capable of processing data sets from human-sized genomes (3Gb) and transcriptomes. Together the new cDNA sequencing protocol and assembler software offer a complete solution for de novo transcriptome sequencing and analysis, enabling a comprehensive picture of the gene space of nearly any organism, even previously uncharacterized species.