A Revolutionary Approach to Peptide Mass Fingerprinting and Nano-Western Blotting
Product News May 30, 2005
Shimadzu Biotech has released the ChIP 1000 Chemical Inkjet Printer, new tool for Proteomics Analysis (peptide fingerprint mapping), Protein Macroarray Analysis and Nano-western Analysis.
Developed in partnership with Proteome Systems Ltd., the ChIP is designed to rapidly and reproducibly dispense picoliter volumes of reagents to defined locations on PDVF membranes, allowing for multiple micro-chemistries on specific western blot spots or bands (from 1 or 2- Dimensional gel electrophoresis), opening new horizons for micro-scale protein research and repeated analysis.
This is accomplished through a piezoelectric reagent delivery system, which includes four independent channels for deposition of multiple chemistries or MALDI-TOF matrices to a stationary support.
Micro-scale on-membrane digestion can be rapidly generated without the time-consuming procedures of in-gel digestion; peptide extraction and C18 clean-up steps.
Shimadzu Biotech's ChIP is a unique tool for accessing proteins separated by gel electrophoresis, and can be used for the study of separated isoforms. It delivers highly localized 'on-membrane' reactions on protein microspots, allows multiple chemistries on a stable, archived support, and allows direct transfer of support to MALDI MS.