AnaSpec Introduces Fluorescent GOÔ Peptides
Product News Jun 19, 2009
Leveraging its dual expertise in peptides and fluorescent dyes, AnaSpec offers a broad selection of fluorescently labeled peptides labeled with our proprietary HiLyte FluorÔ dyes, as well as classic dyes.
Peptide substrates used in detection of enzyme activity can contain either a single dye or in the case of FRET, two dyes. In the intact fluorescent substrates, there is low fluorescence prior to enzyme hydrolysis. Upon recognition of the substrate by a specific enzyme and subsequent cleavage, the quenched fluorescence is recovered.
Increase in fluorescence is correlated to enzyme activity.2-3 AnaSpec’s single dye-labeled fluorescent peptide selection include substrates for kinases, caspases, cathepsins, HDAC (histone deacetylase), calpain, kallikrein, and others. The FRET peptides, generally consisting of a dye (donor) and a non-fluorescent quencher (acceptor), include substrates for MMPs, Aggrecanase-1, HCV, HIV, cathepsins, renin, ACE2, a and ß-secretases and others.