AnaSpec Introduces HiLyte Fluor™ 594 Labeled Secondary Antibodies and Streptavidin
Product News Apr 14, 2009
AnaSpec has introduced a line of secondary antibodies and streptavidin conjugated to the newest member of the HiLyte Fluor™ family - HiLyte Fluor™ 594.
Using optimal fluorophore/protein labeling ratio, these HiLyte Fluor™ 594-IgG conjugates exhibit high fluorescent signal and uncompromised biological function. The fluorescence of HiLyte Fluor™ 594 can be detected with filters designed for Texas Red®.
The labeling performance and stability properties of HiLyte Fluor™ 594 are better than those of Texas Red®. It has a higher extinction coefficient (80,000 M-1cm-1) and a lower correction factor (0.17) compared to Alexa Fluor™ 594 (Invitrogen). The fluorescence quantum yield of HiLyte Fluor™ 594 is 0.9 in aqueous solution.
Biomolecules conjugated to HiLyte Fluor™ 594 exhibit little spectral overlap with green-fluorescent conjugates, and can be efficiently excited by 568 nm line of Ar-Kr laser and by the 594 nm line of orange He-Ne laser.
HiLyte Fluor™ 594 conjugated streptavidin provides high fluorescence intensity and low background as validated in immunofluorescence staining of mammalian cells. HiLyte Fluor™ 594-protein conjugates are suitable for immunofluorescent staining, in situ hybridization, flow cytometry and other biological applications.
The newly developed HiLyte Fluor™ 594 is available in a variety of reactive forms, such as acid, amine, C2 maleimide, hydrazide, succinimidyl ester (SE).