We've updated our Privacy Policy to make it clearer how we use your personal data.

We use cookies to provide you with a better experience. You can read our Cookie Policy here.

Advertisement
AnaSpec Introduces New HiLyte Fluor™ 594 Fluorescent Probe
Product News

AnaSpec Introduces New HiLyte Fluor™ 594 Fluorescent Probe

AnaSpec Introduces New HiLyte Fluor™ 594 Fluorescent Probe
Product News

AnaSpec Introduces New HiLyte Fluor™ 594 Fluorescent Probe


Want a FREE PDF version of This Product News?

Complete the form below and we will email you a PDF version of "AnaSpec Introduces New HiLyte Fluor™ 594 Fluorescent Probe"

First Name*
Last Name*
Email Address*
Country*
Company Type*
Job Function*
Would you like to receive further email communication from Technology Networks?

Technology Networks Ltd. needs the contact information you provide to us to contact you about our products and services. You may unsubscribe from these communications at any time. For information on how to unsubscribe, as well as our privacy practices and commitment to protecting your privacy, check out our Privacy Policy

AnaSpec has introduced the new member of the HiLyte Fluor™ family - HiLyte Fluor™ 594 (Ex/Em=593/616 nm), a bright and red fluorescent probe.

HiLyte Fluor™ dyes are a series of high-performance fluorescent labeling dyes that span the full visible spectrum. These dyes have been optimized to yield maximum brightness when conjugated with macromolecules.

Available in a variety of reactive forms, such as amine-reactive, thiol-reactive and amine-containing derivatives - allowing conjugation to biopolymers.

HiLyte Fluor™ 594 has spectral characteristics similar to those of Texas Red® (a trade mark of Invitrogen for sulforhodamine 101 sulfonyl chloride), with excitation and emission wavelength at 593/615 nm in PBS buffer (pH 7.4), and 596/617 nm when conjugated to macromolecules. It has a higher extinction coefficient (80,000 M-1cm-1) and a lower correction factor (0.17) compared to Alexa Fluor™ 594 (Invitrogen).

The fluorescence quantum yield of HiLyte Fluor™ 594 is 0.9 in aqueous solution. HiLyte Fluor™ 594 conjugated streptavidin provides high fluorescence intensity and low background as validated in immunofluorescence staining of mammalian cells.

Bioconjugates conjugated to HiLyte Fluor™ 594 exhibit little spectral overlap with green-fluorescent conjugates, and can be excited by 568 nm line of Ar-Kr laser and by the 594 nm line of orange He-Ne laser.

HiLyte Fluor™ 594 conjugated proteins have been optimized in a specific fluorophore / protein labeling ratio to ensure a high fluorescent signal and uncompromised protein functions. The HiLyte Fluor™ 594-protein conjugates are suitable for immunofluorescent staining, in situ hybridization, flow cytometry and other biological applications.
Advertisement