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AnaSpec Introduces New HiLyte FluorTM 405 Fluorescent Probe
Product News

AnaSpec Introduces New HiLyte FluorTM 405 Fluorescent Probe

AnaSpec Introduces New HiLyte FluorTM 405 Fluorescent Probe
Product News

AnaSpec Introduces New HiLyte FluorTM 405 Fluorescent Probe


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HiLyte FluorTM dyes are a series of superior fluorescent labeling dyes that span the full visible spectrum. They are optimized for maximum brightness when conjugated with macromolecules.


Available in a variety of reactive forms, such as amine-reactive, thiol-reactive and amine-containing derivatives, HiLyte FluorTM 405 allows for easy conjugation to biopolymers. HiLyte Fluor™ 405 has spectral characteristics similar to those of Alexa Fluor™ 405 (Invitrogen) and DyLight Fluor™ 405 (Pierce) dyes with excitation and emission wavelength at 404/428 nm in PBS buffer (pH 7.4) (see spectra below). Conjugates of HiLyte Fluor™ 405 are well excited by the 407 nm spectral line of the Krypton laser and the 408 nm line of the violet laser diode.


HiLyte Fluor™ 405 conjugates, whether conjugated to primary tag, secondary antibodies or streptavidin, are prepared in an optimized fluorophore/protein labeling ratio to ensure high fluorescent signal and uncompromised biological function. Fluorescence signal can be observed at Ex/Em = 404/428 nm.

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