We've updated our Privacy Policy to make it clearer how we use your personal data.

We use cookies to provide you with a better experience. You can read our Cookie Policy here.

Advertisement
AnaSpec Introduces New SensoLyte® 520 Total GSH Quantitation Assay
Product News

AnaSpec Introduces New SensoLyte® 520 Total GSH Quantitation Assay

AnaSpec Introduces New SensoLyte® 520 Total GSH Quantitation Assay
Product News

AnaSpec Introduces New SensoLyte® 520 Total GSH Quantitation Assay


Want a FREE PDF version of This Product News?

Complete the form below and we will email you a PDF version of "AnaSpec Introduces New SensoLyte® 520 Total GSH Quantitation Assay"

First Name*
Last Name*
Email Address*
Country*
Company Type*
Job Function*
Would you like to receive further email communication from Technology Networks?

Technology Networks Ltd. needs the contact information you provide to us to contact you about our products and services. You may unsubscribe from these communications at any time. For information on how to unsubscribe, as well as our privacy practices and commitment to protecting your privacy, check out our Privacy Policy

After the launch of its SensoLyte® 520 Thiol Quantitation Assay Kit (patent pending), AnaSpec has released the SensoLyte® 520 Total GSH Assay Kit (patent pending).

The SensoLyte® 520 Total GSH Assay Kit provides a sensitive fluorescent assay for the measurement of total GSH (GSH+GSSG). It employs a novel fluorogenic reagent, which upon reaction with reduced GSH releases fluorescence.

Fluorescence is monitored at Ex/Em= 490/520 nm. Additionally, oxidized GSH can be converted to the reduced GSH by glutathione reductase and total GSH level can be quantified. The assay has a linear range of 0.2-100 mM of glutathione. The kit also provides sulfosalicylic acid for deproteination of biological samples.

A colorimetric GSH kit, the SensoLyte® Total GSH Assay Kit is also available. This kit contains Ellman’s Reagent [5, 5’-dithiobis (2-nitrobenzoic acid)], which upon reaction with reduced GSH releases a colored product, monitored at 415 nm using a microplate reader. The intensity of the color produced is proportional to GSH concentration.

Reduced Glutathione (GSH) is the main thiol (sulfhydryl) compound in animal, plant and protozoa. GSH plays a crucial role in cellular defense against oxidative stress and functions as a cofactor for a variety of enzymes such as glutathione transferase and glutathione peroxidase.
Advertisement