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AnaSpec Introduces new SensoLyte® LSD1 Assay Kit
Product News

AnaSpec Introduces new SensoLyte® LSD1 Assay Kit

AnaSpec Introduces new SensoLyte® LSD1 Assay Kit
Product News

AnaSpec Introduces new SensoLyte® LSD1 Assay Kit


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AnaSpec has announced the release of a new long wavelength assay for Lysine-Specific Demethylase –1 (LSD1), the SensoLyte® LSD1 Assay Kit, Ex/Em=530/590 nm.

LSD1 is a histone-modifying enzyme catalyzing lysine demethylation of histone H3. It plays an important role in regulation of gene expression, acting either as a transcription co-repressor or co-activator. The enzyme is implicated in cellular differentiation and has been considered as a potential target for drug discovery.

The SensoLyte LSD1 assay kit provides assay for screening of LSD1 inhibitors and for detection of LSD1 activity. This assay consists of two steps.

In the first step, LSD1 enzyme is incubated with a methylated peptide substrate and hydrogen peroxide (H2O2) is generated. In the second step, in the presence of peroxidase, H2O2 reacts with fluorogenic substrate to produce fluorescence signal, which is monitored at Ex/Em=530/590 nm.
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