AnaSpec Introduces SensoLyte 520 Calpain Kit
Product News Nov 19, 2009
AnaSpec has announced the release of the SensoLyte 520 Calpain Activity Assay Kit, the industry’s first longest wavelength FRET based calpain assay. Optimized for detecting calpain activity, this kit contains a novel internally quenched 5-FAM/QXL™ 520 FRET substrate.
Cleavage of this FRET substrate generates two separate fragments. The subsequent release of 5-FAM fluorescence can be monitored at excitation/emission= 490/520 nm. Increase in fluorescence is proportional to the calpain activity. The assay can detect both calpain 1 (m) and 2 (m) activities and is ideal for kinetic study of these enzymes. The long wavelength fluorescence of 5-FAM is less interfered by the autofluorescence of components in biological samples and test compounds.
An AMC based calpain assay, the SensoLyte® AMC Calpain Assay, is also available from AnaSpec.