We've updated our Privacy Policy to make it clearer how we use your personal data.

We use cookies to provide you with a better experience. You can read our Cookie Policy here.

Advertisement
AnaSpec Introduces Ultra-Sensitive HCV NS3/4A Protease FRET Substrate
Product News

AnaSpec Introduces Ultra-Sensitive HCV NS3/4A Protease FRET Substrate

AnaSpec Introduces Ultra-Sensitive HCV NS3/4A Protease FRET Substrate
Product News

AnaSpec Introduces Ultra-Sensitive HCV NS3/4A Protease FRET Substrate


Want a FREE PDF version of This Product News?

Complete the form below and we will email you a PDF version of "AnaSpec Introduces Ultra-Sensitive HCV NS3/4A Protease FRET Substrate"

First Name*
Last Name*
Email Address*
Country*
Company Type*
Job Function*
Would you like to receive further email communication from Technology Networks?

Technology Networks Ltd. needs the contact information you provide to us to contact you about our products and services. You may unsubscribe from these communications at any time. For information on how to unsubscribe, as well as our privacy practices and commitment to protecting your privacy, check out our Privacy Policy

AnaSpec has developed a tool for optimized detection performance, focusing on the NS3/4A protease.


NS3/4A protease is the protease responsible for the cleavage of HCV nonstructural polyprotein at the NS3-NS4A, NS4A-NS4B, NS4B-NS5A, and NS5A-NS5B sites.


Since these cleavages are essential for the maturation of the viral proteins, this protease has become one of the key targets for developing anti-HCV drugs. Applying both peptide and fluorescent dye technologies, AnaSpec has developed an ultra-sensitive HCV NS3/4A protease FRET substrate that is designed to detect the presence of HCV NS3/4A protease at levels of < 0.1 pmol. 


Based on the peptide sequence, Ac-DED(EDANS)EE-Abu-[y,COO]ASK(DABCYL)-NH2, developed by Taliani, M. et al. (1), the donor and the quencher have been substituted with 5-FAM and our trademark quencher QXLTM 520, respectively.


This substrate, Ac-DE-DAP(QXL520)-EE-Abu-[y,COO]AS-C(5-FAMsp)-NH2, shows a 22-fold lower Km than the EDANS/DABCYL peptide.

Advertisement