BAC BV Launches CaptureSelect® C-tag Affinity Matrix
Product News May 30, 2012
A revolutionary purification resin for C-terminal EPEA tagged proteins combining highly selective binding, even under denaturing condition, with mild elution of the target using the shortest affinity tag system available.
BAC BV’s CaptureSelect C-tag affinity matrix combines the exceptional selectivity for the shortest affinity tag available, E-P-E-A (glutamic acid-proline-glutamic acid-alanine), with a robust and high quality affinity resin.
These properties allow for one-step purification of any C-tagged protein with high purity and yield under both physiological as well as denaturing conditions.
In addition, pH sensitive target proteins can be eluted under mild conditions ensuring high activity of purified protein.
The CaptureSelect C-tag affinity matrix is specifically designed to simplify the workflow of researchers by reducing both the time and cost of purification, whilst delivering higher quality output and increased flexibility in the protein purification process.
Researchers only need to clone the EPEA sequence at the C-terminal end of the protein of interest.
The CaptureSelect C-tag resin provides for a highly specific purification of the tagged protein and is also suitable for use in high throughput processes as well as larger scale packed bed chromatography.
The CaptureSelect C-tag affinity matrix is available alongside detailed protocols and applications at http://www.captureselect.com/C-tag.
Common tag systems all have their limitations, such as HIS or GST which, for example, often lack proper selectivity when purifying proteins from complex mixtures.
The FLAG system provides for excellent specificity, but lacks in stability and is expensive. In addition, both GST and FLAG tags are large in size, which can influence activity of the protein of interest.
None of these systems offers the flexibility to chose between the multiple elution conditions available with the CaptureSelect C-tag resin.
This novel matrix expands BAC BV’s innovative CaptureSelect portfolio of robust affinity products used in the development of biopharmaceuticals.