Highly Reproducible Gene Delivery for Stem Cell Research
Product News May 10, 2010
AMSBIO have announced a range of ready-to-use lentivirus supernatant products suitable for many kinds of gene delivery applications including stem cell research.
Converting fully differentiated mouse or human somatic cells into embryonic-like cells has attracted enormous attention in stem cell research. Multiple reports have demonstrated that iPS cells were generated by using a set of transcription factors or stem cell factors that delivered as expression virus or expressed proteins.
Although the combination of reprogramming factors may slightly different, the main stem cell factors are: OCT3/4, SOX2, NANOG, LIN28, c-Myc and KLF4. iPSC holds the promise of curing many human diseases and accelerates the stem cell research.
Using the SureTiter™ lentiviral system from AMSBIO, you can generate high-titer lentiviral particles for all six human stem cell factors. Each factor was fully sequencing verified and matched to the CDs in NCBI database. High titer lentiviral particles / supernatant were produced in 293T packaging cells in DMEM with 10% heat-inactivated FBS. They are psudotyped with VSV-G glycoprotein-attached vector map.
Prepared using proprietary protocols to integrate a real-time fluorescence monitoring tag in the system - each 200 ul vial of AMSBIO lentivirus supernatent contains a high titre of highly transducible lentivirus (1 x 10 e7 IFU/ml). Each vial of lentiviral supernatent particles contains a fully sequence verified target, ready for transduction into any mammalian cells.
All AMSBIO lentivirus supernatent products are easy and safe to use, simply add 50 ul into the cultured cells, and you will be able to confirm the specific target's expression under a fluorescent microscope after 48-72 hours.