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Myths about Peak Area Response of Glycoprotein Oligosaccharides
Product News

Myths about Peak Area Response of Glycoprotein Oligosaccharides

Myths about Peak Area Response of Glycoprotein Oligosaccharides
Product News

Myths about Peak Area Response of Glycoprotein Oligosaccharides


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Thermo Fisher Scientific has released a technical note (TN) that challenges the common misconception that there is significant variation in high-performance anion-exchange chromatography with pulsed amperometric detection (HPAE-PAD) peak area response of oligosaccharides.

TN 133: HPAE-PAD Peak Area Response of Glycoprotein Oligosaccharides summarizes the peer-reviewed scientific literature concerning this subject.

This review concludes that the variation in HPAE-PAD oligosaccharide response with structure is minimal, especially among sets of similar oligosaccharides such as those found on many glycoproteins.

HPAE-PAD is a key technique used for characterizing recombinant therapeutic glycosylation because it can be used for monosaccharide, sialic acid, and oligosaccharide analyses (Asn-linked [N-linked] and Ser/Thr-linked [O-linked] oligosaccharides)-commonly referred to as oligosaccharide profiling.

In this review of scientific literature evaluating electrochemical response of glycoprotein-derived and related oligosaccharides, TN 133 shows that the changes of electrochemical response with structure are not extreme.

TN 133 then compares HPAE-PAD to other common methods of oligosaccharide profiling, including matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) analysis of oligosaccharides.

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