Profinity Exact Fusion-Tag System Brochure is Available
Product News Apr 12, 2008
Bio-Rad Laboratories, Inc. has announced the availability of Bio-Rad’s Profinity eXact™ Fusion-tag System brochure (bulletin 5668A).
The bulletin provides a snapshot of the Profinity eXact system, which consists of expression vectors, chemi-competent cells, SOC growth media, spin columns, purification resin, cartridges, and detection reagents.
The bulletin demonstrates the one-step purification and tag cleavage mechanism of the system, while comparing it to conventional, more tedious methods of tag removal.
Protein fusion technology has greatly simplified the process of purifying recombinant proteins. Recombinant technology has made the expression of large numbers of proteins possible, usually in tagged format, allowing their use in standard chromatography applications.
The Profinity eXact system addresses the bottlenecks in the tag-removal process during affinity-tagged protein purification. The Profinity eXact system is E. coli-based for the expression, detection, purification, and on-column cleavage of affinity-tagged proteins, without the addition of protease, and offers single-step purification without the challenge and expense of cleavage enzymes, incubation times, or removal of reagents.
Key benefits of the Profinity eXact system include:
• Purification and processing of fusion-tagged proteins in a single step
• On-column cleavage in as little as 30 minutes
• Elimination of a protease addition step
• Precise cleavage at the N-terminus to generate a native protein sequence.